【作者】 付贵权； 韩婷婷； 巩秀玉； 黄洪辉；

【Author】 Fu Gui-quan;Han Ting-ting;Gong Xiu-Yu;Huang Hong-hui;Key Laboratory of Marine Fishery Ecology Environment of Guangdong Province;Key Laboratory of South China Sea Fishery Resources Development and Utilization,Ministry of Agriculture;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;Collage of Fishery and Life Science,Shanghai Ocean University;

【机构】 广东省渔业生态环境重点实验室农业部南海渔业资源开发利用重点实验室中国水产科学研究院南海水产研究所； 上海海洋大学水产与生命学院；

【摘要】 本文采用半静态实验方法研究了半叶马尾藻(Sargassum hemiphyllum)对重金属铜(Cu)胁迫的生理响应。实验设置4个胁迫组,Cu浓度分别为0.01、0.05、0.25和1.25mg/L,不加Cu为对照组,在0d、2d、4d和8d时分别测定藻体鲜重、叶绿素a(Chla)和类胡萝卜素(Car)含量、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性及丙二醛(MDA)含量。结果表明:随Cu浓度增大藻体相对生长速率(RGR)呈先升后降的趋势,其中以0.05mg/L胁迫组为最大;Cu≤0.05mg/L的胁迫组,Chla和Car含量均恢复到对照组水平(P>0.05),SOD活性相对稳定;而Cu>0.05mg/L的胁迫组,胁迫2d后,Chla和Car含量均低于对照组,1.25mg/L胁迫组的SOD活性显著高于对照组(P<0.05),至8d时,CAT活性和MDA含量均显著高于对照组(P<0.05),且1.25mg/L胁迫组的MDA含量显著下降(P<0.05)。因此,当Cu≤0.05mg/L时,在一定程度上可促进藻体生长,抗逆能力无明显变化;而Cu>0.05mg/L时,藻体生长和抗逆能力均降低。更多还原

【Abstract】 In order to study the growth and biochemical composition responses of Sargassum hemiphyllum to Cu stress, the thallis under four treatments with different Cu concentrations: 0.01, 0.05, 0.25 and 1.25 mg/L, together without Cu as the control, were incubated in laboratory with a semi-static test for 8 days. The fresh weight, chlorophyll a(Chla), carotenoid(Car), superoxide dismutase(SOD), catalase(CAT) and malondialdehyde(MDA) of thalli were determined in 0d, 2d, 4d and 8d. The results showed that: with the increasing Cu concentration, the RGR of thalli was increased until Cu concentration was 0.05mg/L, and then decreased. The content of Chla and Car, in Cu≤0.05mg/L groups, were recovered to the control level, SOD activity was relatively stable. However, after 2d, the content of Chla and Car were lower than the control in Cu>0.05mg/L groups, and SOD activity was higher than the control significantly when Cu concentration was 1.25mg/L(P<0.05); and to 8d, CAT activity and MDA content were higher than the control significantly(P<0.05), and the MDA content of 1.25mg/L was dropped significantly(P<0.05). Therefore, it was suggested that the growth of thalli was promoted to some extent and the resilience was no change significantly in Cu≤0.05mg/L; however, it was inhibited and the resilience was weakened in Cu>0.05mg/L.更多还原