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黄颡鱼“溃疡综合征”病原的分离鉴定及致病性研究
Isolation, identification and pathogenicity on Ulcerative Disease Syndrome Pathogen of Pelteobagrus fulvidraco
【作者】 徐洋; 姚嘉赟; 盛鹏程; 潘晓艺; 蔺凌云; 尹文林; 沈锦玉;
【Author】 XU Yang;YAO Jia-yun;SHENG Peng-cheng;PAN Xiao-yi;LIN Ling-yun;YIN Wen-lin;SHEN Jin-yu;Zhejiang Institute of Freshwater Fisheries;
【机构】 浙江省淡水水产研究所;
【摘要】 对湖州地区患"溃疡综合征"的黄颡鱼开展病原研究,从典型濒死的病样体内分离到1株优势菌TH0426,通过人工感染试验成功复制"溃疡综合征"症状,证明该优势菌为黄颡鱼"溃疡综合征"病原菌,其半数致死量(LD50)为3.98×104CFU。对病原菌进行了形态特征、理化特性及分子生物学等方面的综合分析,结果表明该病原菌为维氏气单胞菌(Aeromonas veronii)。药敏试验结果显示头孢曲松、利福平、四环素、多粘菌素B、氟苯尼考、头孢噻吩、强力霉素、头孢西丁、诺氟沙星、头孢拉定、氧氟沙星、环丙沙星、头孢西丁、氟罗沙星和恩诺沙星等15种药物对该菌有较好的抑制作用。
【Abstract】 We studied pathogen on Ulcerative Disease Syndrome(UDS)of Pelteobagrus fulvidraco. A bacterical strain, TH0426, was isolated from diseased Pelteobagrus fulvidraco. An artificial infection test showed that TH0426 was the pathogen on(UDS)of Pelteobagrus fulvidraco and the LD50 was about 3.98×104CFU. Based on its morphological, physiologial and biochemical charateristics and 16 S r DNA molecular analysis were carried out for the strain TH0426 was Aeromonas veronii. The drug sensitivty test indicated that TH0426 was susceptible to 15 k inds of antibiotics, including Ceftriaxone Sodium, rifampicintablets, tetracycline, Polymyxin B sulfate, florfeniol, ceftofur, doxycycline, norfloxacin, cefradex, ofloxacin, ciprofloxacin, cefoxitin, feroxacin and enrofloxacin.
【Key words】 Pelteobagrus fulvidraco; Ulcerative Disease Syndrome(UDS); Pathogen; Aeromonas veronii; Isolation and identification;
- 【会议录名称】 2014年中国水产学会学术年会论文摘要集
- 【会议名称】2014年中国水产学会学术年会
- 【会议时间】2014-10-29
- 【会议地点】中国湖南长沙
- 【分类号】S943
- 【主办单位】中国水产学会