【作者】 古川； 梁艳妮； 武婧； 刘红波； 唐志书；

【Author】 GU Chuan;LIANG Yanni;WU Jing;LIU Hongbo;TANG Zhishu;Shaanxi University of Chinese Medicine/Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Shaanxi province key laboratory of new drugs and Chinese medicine foundation research;

【机构】 陕西中医学院陕西省中药资源产业化协同创新中心陕西省中药基础与新药研究重点实验室；

【摘要】 目的:研究不同纯化技术对甘草水提液的纯化效果.方法:分别采用Ⅱ型ZTC 1+1天然澄清剂、壳聚糖、微滤与DA—201型大孔吸附树脂联用,以及微滤与超滤联用等4种技术对甘草水提液进行分离纯化,以指标性成分的保留率、物理化学参数及HPLC指纹图谱等指标综合分析以上方法的适用性.结果:联用技术会使指标性成分的含量降低,但有效去除了大分子物质.其中微滤技术粗纯化除杂效果最好,微滤与超滤联用得到的纯化液与原液的相似度为0.886,在4种纯化技术中对药液组分影响最小.结论:微滤与超滤联用技术或许是较好的中药水提液分离纯化方法,该方法避免了有机试剂对中药水提液的污染,操作简便,节省成本,适合工业化连续生产.更多还原

【Abstract】 Objective:To explore the effects of different purification technologies on water extract from Glycyrrhiza.Method:Four kinds of technologies of ZTCl +1 Ⅱ natural clarifying agents,chitosan and microfiltration combined with DA- 201 macroporous absorbent resin respectively,in addition microfiltration-ultrafiltration were all used for water extract purification from Glycyrrhiza.The retention rates of the index components,physical and chemical parameters,and HPLC fingerprint were all applied to analyse the applicability of different technologies.Result:The index components were reduced with combination technologies,which effectively removedmacromolecules in the water extract at the same time.The microfiltration technology in removing impurity is the best among the primary purifications.The similarity of purified liquid with the microfiltrationTtrafiltration technology compared with original liquid is0.886,which had minimal effect on the liquid components among four purification technologies.Conclusion;The microfiltrationTtrafiltration combination technology might be a better method for separation and purification of water extract of Chinese medicines.This method avoids the pollution of organic reagents on water extract of Chinese medicines,which is suitable for industrialized continuous production for easy operating and cost saving.更多还原