【作者】 肖珺； 邹潇潇； 黄惠琴； 孙前光； 刘敏； 鲍时翔；

【Author】 XIAO Jun;ZOU Xiao-xiao;HUANG Hui-qin;SUN Qian-guang;LIU Min;BAO Shi-xiang;Institute of Tropical Bioscience and Biotechnology,CATAS;

【机构】 中国热带农业科学院热带生物技术研究所；

【摘要】 穿刺巴斯德芽菌(Pasteuria penetrans)是根结线虫的专性寄生菌,是最有潜力的根结线虫生防因子。胡椒作为海南的主要经济作物之一,根结线虫发病率较高。前期的研究发现,在海南岛胡椒中较易分离到P.penetrans。结合形态学和16S rDNA序列分析的方法,对海南岛6个市县共13个胡椒园进行P.penetrans分离与鉴定,在经筛选获得的33个具代表性巴斯德芽菌克隆中,32个克隆子被鉴定为P.penetrans,而克隆子tf-1与P.penetrans~T(AF077672)相似性仅为97.7%,且在发育树上处于独立分支,可能为巴斯德芽菌属的一个新种。经系统发育分析,海南岛存在较丰富的P.penetrans资源,但在不同的地理区域内没有发现明显的种内地域特异性。更多还原

【Abstract】 Pasteuria penetrans is an obligate parasite in root-knot nematodes and also described as the most potential biopesticide.Pepper is one of the main economic crops in Hainan Island and the incidence of root-knot nematode diseases on pepper is fairly high.Based on the previous studies,P.penetrans resources were easier to be discovered on pepper than other plants in Hainan Island.Through the combination of morphological identification and the 16S rRNA gene sequence analysis,the Pasteuria spp.resources from 13 pepper fields located in 6 cities of Hainan Island were investigated and identified.Thirty-three representative Pasteuria clones were screened out,and thirty-two of them were identified as P.penetrans.Though the clone tf-1 was closely related to Pasteuria penetrans~T(AF077672),the sequence similarity was only 97.7% and formed a single branch in the phylogenetic tree,which suggested that tf-1 might be a novel species in the genus Pasteuria.The phylogenetic analysis showed that there were abundant P.penetrans resources in Hainan Island,but no obvious geographic specificity existed in distinct regions within the P.penetrans species.更多还原