【作者】 张全才； 唐青涛； 黄延盛； 吴小勇；

【Author】 ZHANG Quan-cai;TANG Qing-tao;HUANG Yan-sheng;WU Xiao-yong;Infinitus(China) Company Ltd;School of Food Science,Guangdong Pharmaceutical University;

【机构】 无限极(中国)有限公司； 广东药学院食品科学学院；

【摘要】 本研究采用水提法提取多糖,以葡聚糖为标准,采用蒽酮-硫酸法测定样品多糖含量,考察了液料比、搅拌转速、提取时间、提取温度对灵芝粗多糖得率的影响,并对灵芝多糖提取工艺进行了优化。结果表明:影响灵芝多糖得率的因素排序为,液料比>提取温度>搅拌转速>提取时间。优化得到灵芝多糖提取的最佳工艺条件为:将灵芝粉碎成木屑状,然后在液料比为20:1,转速为150 r/min,温度为95℃的条件下,搅拌提取110 min。在此最佳工艺条件下,试验用到的灵芝的粗多糖得率平均值为4.33%。更多还原

【Abstract】 In this paper,the water extraction conditions of polysaccharide from dried fruiting bodies of Ganoderma lucidum(G lucidum) were investigated by single-factor test and orthogonal test respectively.The influence factors including liquid-solid ratio,stirring speed,extraction time,and extraction temperature.The optimal condition was obtained,based on the best yield rate of the polysaccharide.Results showed that the most important factor was the liquid-sohd ratio,follow by extraction temperature,then to stirring speed and last by extraction time.The optimal parameters for this study were the solid-liquid ratio of 20:l(mL/g),the stirring speed of 150 r/min at 95℃ for 110 min.Under this condition,the polysaccharide yield rate of the G.lucidum could up to be 4.33%.更多还原