【作者】 葛宇； 李雪； 杨晓霞； 徐文龙； 崔崇士； 屈淑平；

【Author】 GE Yu;LI Xue;YANG Xiaoxia;XU Wenlong;CUI Chongshi;QU Shuping;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Department of Horticulture,Northeast Agricultural University;Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences;

【机构】 东北农业大学园艺学院； 中国热带农业科学院海口实验站；

【摘要】 以印度南瓜‘98-2-351’与‘06820-1’杂交构建F₂群体,对亲本及各世代群体成熟果实果皮和果肉颜色进行调查、统计分析。结果表明:F₂群体中果皮桔红色和灰色的分离比呈3:1,这说明果皮灰色是由单隐性基因控制;F₂群体中果肉黄色和白色的分离比呈3:1,这说明果肉白色也是由单隐性基因控制。利用群体分离分析法结合隐性群体分析法,采用SSR分子标记,找到了2个与控制灰色果皮基因位点CmRc紧密连锁的SSR标记,PU078072和PU013839,其连锁遗传距离分别为5.9 cM和14.5 cM;同时找到了1个与控制白色果肉基因位点CmFc紧密连锁的SSR标记PU132712,其连锁遗传距离为6.7 cM。本研究为进一步筛选与控制印度南瓜果皮和果肉颜色基因更加紧密连锁的分予标记及相关基因的精确定位奠定基础。更多还原

【Abstract】 The F₂ population was derived by crossing two diverse Cucurbita maxima lines ’98-2-351’ and’06820-1’.Investigation and statistical analyses were conducted on rind and flesh parental plants and each generation.The results demonstrated that the orange and grey rind plants in F₂population were fitted for the ratio of 3:1,indicating that the grey rind was controlled by a single recessive gene;similarly,the yellow and white flesh plants in F₂ population were also fitted for the ratio of 3:1,showing that the white flesh was controlled by a single recessive gene.With bulked segregation analysis and recessive-class approaches,two SSR markers PU078072 and PU013839 were detected to be tightly linked to the locus CmRc at a distance 5.9 and 14.5 cM,respectively.Meanwhile,one SSR marker PU132712 was founded to be closely linked to the locus CmFc at a distance 6.7 cM.This study laid the foundation for further screening the molecular markers closer linked to rind and flesh color genes of Cucurbita maxima and locating them accurately.更多还原