【作者】 孙晓梅； 崔金秋； 李敏； 周文强； 杨宏光； 王丹；

【Author】 SUN Xiao-mei;CUI Jin-qiu;LI Min;ZHOU Wen-qiang;YANG Hong-guang;WANG Dan;College of Forestry,Shenyang Agricultural University;Shenyang Botanical Garden;

【机构】 沈阳农业大学林学院； 沈阳市植物园；

【摘要】 以芍药种子为试材,采用不同浓度GA₃处理,对种子生根过程中酶的活性变化进行了研究,获得生根过程中各种酶的活性变化规律,为进一步研究种子萌发的分子调控机理奠定基础。结果表明,GA₃能有效打破芍药种子下胚轴休眠。GA₃处理后芍药种子生根过程中SOD、POD、CAT、MDA比未处理的在沙藏第28d时差异达到最大,PAL和蔗糖转化酶活性在沙藏第14d时差异达到最大;不同浓度GA₃处理后,沙藏20d的种子以GA₃ 500mg/L处理的SOD活性最强、MDA含量较低,较适合种子萌发。更多还原

【Abstract】 After GA₃ treatment,enzyme activity was determined in the process of seeds rooting in Paeonia lactiflora,being to abtain various enzyme activity change rule,for laying a foundation for further study of molecular regulation mechanism of seed germination.The results showed that,GA₃ could break dormancy.After GA₃ treatment,SOD,POD,CAT,MDA reached the maximum difference in sand 28 d than no treatment,PAL and invertase reached the maximum difference in sand 14 d.After treated with different concentrations of GA₃,when GA₃ was 500mg/L,the SOD activity of seeds that hidden 20 d reached the strongest,MDA content is low,suitable for seed germination.更多还原