【作者】 王克凤； 桑瀚旭； 董然； 顾德峰； 赵和祥；

【Author】 WANG Ke-feng;SANG Han-xu;DONG Ran;GU De-feng;ZHAO He-xiang;Changchun University of Science and Technology;Jilin Agricultural University;

【机构】 长春科技学院； 吉林农业大学；

【摘要】 以槭叶草叶片、叶柄、带节点的花莛、嫩芽、根芽及种子等为外植体,MS为基本培养基,通过升汞灭菌及在基本培养基内添加不同种类和浓度的激素,筛选出槭叶草组织培养的最佳外植体和最适培养基。结果表明:槭叶草种子为组织培养的最佳外植体;最适槭叶草种子萌发的培养基为MS+6-BA0.5mg/L,其萌发天数最短为4天,萌发率为96.2%;不定芽增殖的最适培养基为MS+6-BA1mg/L+NAA0.1mg/L,繁殖系数高达5.1。更多还原

【Abstract】 The experiment was based on MS culture media and the explants were from leaf、 peeiolts、 scape with node、sprout、 radical bud and seedlings of Mukdenia rossii（Oliv.） Koidz.Using the 1 ‰ HgCl₂ and appropriate culture medium what was obtained via regulating the concentrations of cytokinin and auxin to choose the best explants and tissue culture.Seedlings of Mukdenia rossii（Oliv.） Koidz was the best explantj The best medium to germinat was Ms + 6-BA0.5mg/L The germination duration was 4 days.The germination rate was 96.2%.The Propagation Coefficient of 5.1 times indicated that MS + 6-BA0.1mg/ L + NAA0.1 mg/ L was the optimal culture medium for subculture proliferation.更多还原