【作者】 王轲； 石俊； 鞠易倩； 蔡明； 潘会堂； 张启翔；

【Author】 WANG Ke;SHI Jun;JU Yi-qian;CAI Ming;PAN Hui-tang;ZHANG Qi-xiang;Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding,National Engineering Research Center for Floriculture and College of Landscape Architecture,Beijing Forestry University;

【机构】 花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,北京林业大学园林学院；

【摘要】 以黑钻石系列紫薇品种(‘Pure White’、‘Best Red’、‘Blush’、‘Crimson Red’、‘Red Hot’)的幼嫩茎段为外植体,采用单因子实验方法,通过对茎段进行表面消毒、丛生芽诱导增殖、生根、移栽技术等环节的研究,明确各个阶段的最佳培养基类型和环境条件,建立一套完整的黑钻石紫薇系列组培快繁体系。结果表明:黑钻石系列紫薇品种茎段用75%酒精灭菌30s,0.1%升汞灭菌8min,效果最佳;最佳丛生芽诱导培养基为WPM+0.5mg/L 6-BA+0.05mg/L IBA,丛生芽的平均增殖系数为8.53;最佳生根培养基为1/2MS+0.5mg/LIBA+0.5g/LAC,生根率达到100%。更多还原

【Abstract】 In vitro culture system of Lagerstroemia indka ’Pure White’,’ Best Red’,’ Blush’,’ Crimson Red’ and ’ Red Hot’ were established using soft shoots as explants with single factor experimental design.The effects of sterilization method,media types,plant growth regulator formula and culture condition on shoot multiplication and rooting were investigated.The results showed that the optimal sterilization method was to immerse the shoot in 75%ethanol for 30 seconds,followed by 8 minutes in a 0.1%HgCl₂.The optimal medium for shoot multiplication was WPM + 0.5mg/L6-BA + 0.05mg/L IBA,with multiplication coefficient of 8.53.The optimal rooting medium was 1/2MS + 0.5mg/LIBA + 0.5g/LAC,and the rooting rate was 100%.更多还原