Dynamic physiology and protein profile changes in maize seedlings in response to progressive drought stress

【作者】 邵瑞鑫； 郑会芳； 李蕾蕾； 杨青华；

【Author】 Shao RX;Zheng HF;Li LL;Yang QH;Collaborative Innovation Center of Henan Grain Crops,Agronomy College of Henan Agricultural University;

【机构】 Collaborative Innovation Center of Henan Grain Crops,Agronomy College of Henan Agricultural University；

【摘要】 Drought stress is one of the important environment stresses that greatly limit plant growth and crop production. Maize is considered as one of the most drought sensitive crops, especially, drought stress is easy to occur frequently at maize seedling stage. Therefore, the study on drought adaption mechanism of maize seedlings was investigated using physiological measurements combined with proteomics. In the present study, the third fully expanded leaves were treated with 20% PEG-6000 for 0,1,3 and 5 days,respectively, biomass,osmoticum(such as malonaldehyde(MDA),soluble sugar and proline), photosynthetic parameters and proteomic was determined.The results show that compared with control, after drought stress for 1, 3 and 5 d5 leaf dry weight significantly decreases at the values of 10.2%, 15.0% and 17.0%,respectively; Absolute water content(AWC) decreases of 2.5%, 2.6% and 3.3%;Photosynthetic rate severely decreased. The SOD activity and soluble sugar increased in initial stage and then declined, and the SOD activity increased 187.10%, 235.35%,13.1%, respectively; Soluble sugar increased 21.7%, 30.0% and 10.7%, respectively;The MDA content significantly increased 14.8%, 58.1% and 77.8%; especially, the content of proline markedly increased 0.38,4.2,11.0 times, respectively.Stress-induced proteomics changes were analyzed by 2-DE in conjunction with MALDI-TOF/TOF. 55, 89, 98 spots differed significantly between control and drought(1, 3, 5 days), with 13 up-regulated, and 42 down-regulated spots at 1st drought stress day, 18 up-regulated, and 71 down-regulated at the 3 id drought stress day, 22 up-regulated, and 76 down-regulated at the 5th drought stress day. All of the differentially expressed protein spots were involved in photosynthesis, stress defense,carbohydrate metabolism, energy production, protein metabolism under drought stress conditions. Based on physiological and proteomics measurements, the results help to clarify the physiological and molecular responses of maize seedling to difference drought stress and may provide an insight into the mechanisms underlying drought stress tolerance.更多还原

【Abstract】 Drought stress is one of the important environment stresses that greatly limit plant growth and crop production. Maize is considered as one of the most drought sensitive crops, especially, drought stress is easy to occur frequently at maize seedling stage. Therefore, the study on drought adaption mechanism of maize seedlings was investigated using physiological measurements combined with proteomics. In the present study, the third fully expanded leaves were treated with 20% PEG-6000 for 0,1,3 and 5 days,respectively, biomass,osmoticum(such as malonaldehyde(MDA),soluble sugar and proline), photosynthetic parameters and proteomic was determined.The results show that compared with control, after drought stress for 1, 3 and 5 d5 leaf dry weight significantly decreases at the values of 10.2%, 15.0% and 17.0%,respectively; Absolute water content(AWC) decreases of 2.5%, 2.6% and 3.3%;Photosynthetic rate severely decreased. The SOD activity and soluble sugar increased in initial stage and then declined, and the SOD activity increased 187.10%, 235.35%,13.1%, respectively; Soluble sugar increased 21.7%, 30.0% and 10.7%, respectively;The MDA content significantly increased 14.8%, 58.1% and 77.8%; especially, the content of proline markedly increased 0.38,4.2,11.0 times, respectively.Stress-induced proteomics changes were analyzed by 2-DE in conjunction with MALDI-TOF/TOF. 55, 89, 98 spots differed significantly between control and drought(1, 3, 5 days), with 13 up-regulated, and 42 down-regulated spots at 1st drought stress day, 18 up-regulated, and 71 down-regulated at the 3 id drought stress day, 22 up-regulated, and 76 down-regulated at the 5th drought stress day. All of the differentially expressed protein spots were involved in photosynthesis, stress defense,carbohydrate metabolism, energy production, protein metabolism under drought stress conditions. Based on physiological and proteomics measurements, the results help to clarify the physiological and molecular responses of maize seedling to difference drought stress and may provide an insight into the mechanisms underlying drought stress tolerance.更多还原