【作者】 曾诚； 宓穗卿； 罗颂平； 黄天来； 洪馨； 骆运晖；

【Author】 ZENG Cheng1, MI Suiqing2, LUO Songping1, HUANG Tanglai2, HONG Xin2, LUO Yunhui1(1.The First affiliated hospital, Guangzhou University of TCM, Guangzhou, 510405; 2.Institute of Clinica Pharmacology,Guangzhou University of TCM,Guangzhou 510505)

【机构】 广州中医药大学第一附属医院； 广州中医药大学临床药理研究所；

【摘要】 目的建立菟丝子中槲皮素的含量测定方法,研究槲皮素体内动力学规律。方法建立快速敏感的高效液相色谱-紫外检测法(HPLC-UV),以卡马西平为内标,以菟丝子灌胃给药后,大鼠含药血清样品经液-液萃取后在C18反相色谱柱上进行分离测定。结果槲皮素血药浓度在0.05～9μg/mL浓度范围内线性关系良好(r=0.9998)。方法回收率为86.65～99.07%,提取回收率为79.91～84.56%,最低检测限为0.05μg/mL。结论槲皮素在大鼠体内的动力学过程表现为具吸收的二室开放模型,其中t1/2(Ab)=0.13h,t1/2(α)=0.19h,t1/2(β)=1.22h,tmax=0.333h。实验结果可为菟丝子在临床用药的安全性和有效性提供依据。更多还原

【Abstract】 Objective To establish the method of measuring quercetin content in semen cuscutae(SC) and to explore its in- vivo pharmacokinetics features.Methods The rapid and sensitive HPLC- UV method was adopted with carbamazepine acting as internal standard. After the extract of SC was given, serum samples of rats were collected and measured on C18 reverse- phase chromatographic column after liquid- liquid extract. Results The linear range of calibration curve were 0.05～ 9μ g/mL (r=0.9998). The method showed good precision and recoveries for serum with 86.65～ 99.07% , and extracting recoveries 79.91～ 84.56% .The limit of detection(LOD) as 0.05μ g/mL. Conclusion The pharmacokinetic process of quercetin in vivo manifestated an opening two- compartment model with t1/2(Ab)=0.13h, t1/2(α )=0.19h, t1/2(β ) =1.22h, tmax=0.333h. The results may provide evidence of safety and effectiveness for clinical use of SC.更多还原