【作者】 袁冬平； 龙军； 方泰惠；

【Author】 YUANG Dongping1, LONG Jun2, FANG Taihui1 (1. Department of Pharmacology, Nanjing University of Traditional Chinese Medicine ,Nanjing 210029; 2. China Pharmaceutical University, Nanjing 210009)

【机构】 南京中医药大学药理教研室； 中国药科大学生物制药学院；

【摘要】 目的探讨舒络粉针剂对PC12细胞缺糖、缺氧损伤模型的影响。方法用连二亚硫酸钠(Na2S2O4)、无糖Earle’s液复制PC12细胞缺氧、缺糖模型,通过MTT染色和乳酸脱氢酶(LDH)活性的测定研究舒络粉针剂对PC12细胞的保护作用。结果含舒络血清不同程度的抑制Na2S2O4、无糖Earle’s液造成的缺氧、缺糖样损伤,降低LDH的漏出。结论舒络粉针剂对缺氧、缺糖引起PC12细胞损伤具有保护作用。更多还原

【Abstract】 Objective To investigate the effects of the serum contained Shuluo sterile powder for injection (SLI) on injury induced by anoxia and hypoglycemia in cultured PC12 cells. Methods Cultured PC12 cells were treated with Na2S2O4 and Earle′ s liquid with glucose free to induce models of anoxia and hypoglycemia. The protective effects of the serum contained SLI on the two kinds of models were evaluated by the activity of lactate dehydrogenate (LDH) and staining with MTT assay. Results The serum contained SLI counteracted the injury induced by anoxia and hypoglycemia to various degrees and prevent the efflux of LDH. Conclusion The serum contained SLI can protect PC12 cells from injury induced by anoxia and hypoglycemia.更多还原