【作者】 邓慧敏； 张珍英； 邓芹英； 赵善楷；

【Author】 DENG Hui-min, ZHANG Zhen-ying, DENG Qin-ying, ZHAO Shan-kai (Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou 510275, China)

【机构】 中山大学测试中心；

【摘要】 <正> 高效基质的发现和使用能够提高基质辅助激光解吸/电离(MALDI)的测定效果、扩展其应用范围。自基质辅助激光解吸/电离飞行时间质谱(MALDI-TOFMS)建立以来,寻找有效新基质一直都是MALDI-TOFMS研究的重要内容。目前测定多肽、蛋白质的常用基质主要是一些含苯环、羟基、氨基和羧基等官能基团的有机小分子化合物如SA和HCCA等。本工作拟设计、合成3-氨基(3-AC)、3-羟基(3-HC)、3-羧基(3-CC)和4-甲基-7-羟基香豆素(4-M-7-HC),并考更多还原

【Abstract】 Four coumaric derivatives including 3-aminocoumarin, 3-hydroxycoumarin, 3-carboxycoumarin and 4-methyl-7-hydroxycoumarin were tested as matrices for peptides and proteins analyses by matrix-assisted laser desorption/ionization time-of-flighf mass spectrometry (MALDI-TOFMS). The experimental results showed that all these coumaric derivatives (dissolved in acetonitrile at the concentration of 0.1mol/L) can desorb/ionize the peptide and protein samples effectively and high resolution MALDI-TOFMS spectra were obtained.更多还原