【作者】 刘丹； 陈笑艳； 顾琦； 王英武； 钟大放；

【Author】 LIU Dan~1, CHEN Xiao-yan~1, GU Qi~1, WANG Ying-wu~2, ZHONG Da-fang~(1,2,*)(1. Laboratory of Drug Metabolism and Pharmacokinetics,Shenyang Pharmaceutical University, Shenyang 110016, China;2. Research Center of Drug Metabolism, Jilin University, Changchun 130023, China)

【机构】 沈阳药科大学药物代谢与药物动力学实验室； 吉林大学药物代谢研究中心；

【摘要】 <正>佐米曲普坦为新型、高效、选择性5-HT_1B/1D受体激动剂,同时具有外周和中枢作用,并可作用于中枢的三叉神经核,可有效缓解偏头痛。文献报道多以固相萃取法提取生物样品中的佐米曲普坦,这些方法对佐米曲普坦的定量下限仅为1.0和2.0ng/mL。本工作旨在建立一种快速、灵敏的LC-MS/MS法测定人体血浆中的佐米曲普坦,用于该药药物动力学研究。更多还原

【Abstract】 A rapid and sensitive liquid chromatography-tandem mass spectrometry method was devel- oped and validated for determination of zolmitriptan in human plasma. The analytes were extracted from plasma samples by liquid-liquid extraction, separated on a Zorbax Extend-C₁₈ column and de-tected by tandem mass spectrometry with a TurboIonSpray ionization interface. Diphenhydramine was used as the internal standard. The standard calibration curve for zolmitriptan was linear in the concentration ranges 0.05～30 ng/mL in human plasma. The intra- and inter-run precisions were less than 10%, determined from QC samples for zolmitriptan, and accuracy was within ±3.5%. The method was successfully applied for the pharmacokinetics of zolmitriptan in 24 volunteers after oral dose of 5 mg zolmitriptan.更多还原