【作者】 谢智勇； 孙玉明； 陈笑艳； 钟大放；

【Author】 XIE Zhi-yong, SUN Yu-ming, CHEN Xiao-yan, ZHONG Da-fang (Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang 110016, China)

【机构】 沈阳药科大学药物代谢与药物动力学实验室；

【摘要】 <正>泮托拉唑(Pantoprazole)是国内外临床应用较广的安全、高效的抗消化道溃疡药物。临床数据表明,其口服吸收快,但不规则,吸收入血后与血浆蛋白的结合率为97%左右,个体差异显著,与体内的羟基化酶CYP450 2C19的遗传多态性有关。虽然有其体外代谢研究的报相关道,但很少有其在体内代谢研究的文献报道。本工作旨在应用高灵敏度、高选择性的高效液相色谱-离子阱质谱联用技术鉴定大鼠尿中的代谢物,为更多还原

【Abstract】 Fouteen urinary metabolites of pantoprazole in Wistar rats 0～12 h urine samples after administration of 10 mg/kg pantoprazole) were identified by LC-ESI/MS~n. The mixed urine was simply enriched by Cis SPE column, pantoprazole and its metabolites were completely separated by HPLC and detected in selected ion monitoring (SIM) mode by ESI-MS~n. Product ion spectra of molecular ions were recorded after collision-induced dissociation by ion trap in full scan MS~2 and full scan MS~3 mode. Therefore, fouteen urinary pantoprazole metabolites were identified interpreting mass spectrometric data.更多还原