【作者】 邸宏； 田兴亚； 陈伊里； 卢翠华；

【Author】 Di Hong Tiang Xingya Chen Yili Lu Cuihua (Northeast Agricultural University,Harbin,150030)

【机构】 东北农业大学农学院；

【摘要】 以马铃薯品种 Disiree,东农303和克新4号的叶片和茎段为外植体, MS 为基本培养基,在愈伤诱导培养基中设置不同 NAA 浓度（0．3,2．0, 4．0,8．0mg/L）,分化培养基中分别添加6-BA、GA3,ZT 不同浓度的组合, 进行再生系统的筛选。结果表明,叶片外植体最适的再生系统为 MS+ 2．25mg/L 6-BA+4mg/LNAA→3周后→MS+2．25mg/L6-BA+2mg/LZT+ 5mg/LGA₃,茎段外植体为 MS+2．25mg/L6-BA+4mg/LNAA→3周后→MS +0．3mg/LGA₃,。叶片外植体分化率高于茎段外植体。更多还原

【Abstract】 On MS medium supplemented with various combination of phytohormones, leaf and stem segment taken from three cultivars of Solanum tuberosum L.were tested with their regeneration following a callus phase.The optimized media systems of regeneration for leaf and stem organ S were summarized①MS+2.25mg/L 6-BA+4mg/L NAA→3Weeks →MS+2.25mg/L 6-BA+2mg/L ZT+5mg/L GA₃;②MS+2.25mg/L 6-BA+4mg/L NAA→3Weeks→MS+0.3GA₃ respectively.The regeneratio frequency of leaf segment was higher.更多还原