【作者】 陆绍红； 林爱芬； 陈睿； 闻礼永； 陈彩华； 程永璋； 陈学军； 朱冠；

【Author】 Lu Shao-hong,Lin Ai-fen,Chen Rui,et al (Institute of Parasitic Disease,Zhejiang Academy of Medical Seiences,Hangzhou,310013)

【机构】 浙江省医学科学院寄生虫病研究所； 浙江大学附属儿童医院； 美国纽约州卫生署研究中心传染病处；

【摘要】 目的对浙江省腹泻儿童进行隐孢子虫的病原学诊断及感染情况分析。方法从浙江大学附属儿童医院收集腹泻儿童粪便548份,每份样本先用改良抗酸染色法检查卵囊,发现阳性或可疑标本,再用免疫荧光染色法进一步诊断。结果两种染色方法结合诊断共发现57例阳性病人,隐孢子虫感染率为10．4％,男女之间感染率无显著性差异(X2=007,P<0．05),其中1岁以下婴儿感染率为5．7％,1-10岁儿童感染率为17．5％,两者之间存在着显著性差异(X2=19．69,P<0．05)。结论改良抗酸染色法与免疫荧光染色法结合诊断能达到隐孢子虫确诊目的。由感染情况分析可得浙江省腹泻儿童中有较高的隐孢子虫感染率,其中1岁以下儿童感染率较1岁以上儿童明显降低,可能与1岁以下婴儿多母乳喂养有关．更多还原

【Abstract】 Objective To etiological diagnoes and analyze of gryptosporidium infection of diarrhea children in Zhejiang province.Methods 548 fecal specimens were collected from diarrhea children in affiliated children hospital of Zhejiang college.Each fecal specimen was smeared and screened by modified acid-fast staining method for oocyst;the positive and suspected specimens were identified by immunofluorescent staining method.Results As a result,57cases of infection were found by modified acid-fast staining and immunofluorescent staining method with a positive rate of 10.4%(57/548).Among the positive specimens 19 cases were under the age of 1 year,the positive rate was 5.7%(19/332),and 38 cases were between the age 2 and 10 with a positive rate of 17.5%(38/216). Conclusion The best result could be obtained when modified acid-fast staining method was first used and followed by immunofluorescent staining method.The study showed that there were high Cryptosporidium infective rate in diarrhea children of Zhejiang province,but the positive rate under the age of 1 year was much lower than older children,this may be due to breast feeding.更多还原