【作者】 郑高利； 张信岳； 金锋； 陈凯； 龚维桂； 郑筱祥；

【Author】 Zheng Gao-li, Zhang Xin-yue, Jin Feng,et al (Instituteof Metaria Medico, Zhejiang Academy of Medical Sciences, Hangzhou, 310013)

【机构】 浙江省医学科学院药物研究所； 浙江大学生物医学工程与仪器科学学院；

【摘要】 目的研究葛根总异黄酮(TIP)对去卵巢引起的骨质疏松症大鼠骨骼骨矿和微量元素含量的影响。方法 52只雌性SD大鼠分为假手术、模型、尼尔雌醇(CEE3)、TIP 100和TIP20共5组。除假手术组外均切除双侧卵巢,7d后分别灌胃给予0．5％CNC—Na(假手术和模型组)、CEE3 1．0 mg/kg·wk-1,TIP 100和20mg／kg·d-1,连续7 个月。化学法测定胫骨钙和磷含量,原子吸收分光光度法测定铁、锌、镁、锰、铜、钴、铬和钼等微量元素的含量。结果大鼠在去卵巢后胫骨的表观密度及灰密度分别下降了5．8％和11．4％,钙、磷、铁、锌和镁等元素的含量也显著减少。经TIP 100和20mg/kg·d-1治疗7个月后,胫骨表观密度增加7．5％和7．1％,灰密度增加9．6％和 4．6％;钙密度增加9．8％和6．1％。磷密度增加10．1％和6．2％,对钙磷比值无明显影响。铁、锌和镁含量恢复到接近正常水平,锰和钼含量略高于正常组,而铜比正常组高39％和75％,铬比正常组高313％和265％,钴含量则无明显变化。结论 TIP对雌激素缺乏引起的骨矿和微量元素丢失具有明显的防治作用。更多还原

【Abstract】 Objective The present investigation was to observe the effects of TIP on the contents of mineral and trace elements of tibia in osteoporosis rats induced by ovariectomized. Methods 52 female SD rats were allocated into 5 groups,SHAM, OVX, CEE3, TIP 100 and TIP 20. Except rats in SHAM group which were made sham-operation, the other rats were bilaterally o-variectomized. 7 days after operation, the rats were orally administrated 0.5% CMC-Na(SHAM and OVX),niIestrol(CEE3) 1.0mg/kg·wk-1, TIP 100mg/kg·d-1 (TIP 100) and TIP 20mg/kg·d-1 (TIP 20) separately for 7 months. The contents of calcium and phosphate in tibias were determined by chemical analysis and the trace elements were detected by atom absorption spectrophotometer. Results The apparent density and ash density of tibia in OVX rats were reduced by 5.8% and 11.4% than those in SHAM. Ca, P, Fe, Zn and Mg were also significantly decreased. The apparent density and ash density of tibia in TIP 100 group were increased by 7.5% and 9.6% than OVX group, while by 7.1% and 4.6%in TIP 20 group. Ca and P contents were increased by 9.8% and 10.1% in TIP 100, while by 6.1 % and 6.2% in TIP 20. No markedly change was observed in Ca/ P ratios. The contents of Zn, Fe, Mg, Mn and Mu were slightly raised. Moreover Cu and Cr were even increased by 75% and 300% approximately. Conclusion TIP can inhibit loss of mineral and trace elements in bone induced by estrogen-deficiency. The estrogen-like action of TIP may pay a main role in this protection.更多还原