【作者】 洪艳； 杨连华； 陈勇； 经络； 蒋骏航； 王怡婷；

【Author】 Hong Yan,Yang Lian-hua,Chen Yong,et al (Institute of Bioengineering,Zhejiang Academy of Medical Sciences, Hangzhou,310013)

【机构】 浙江省医学科学院生物工程研究所；

【摘要】 目的探讨含有单纯疱疹病毒Ⅱ型(HSV-2)糖蛋白D全基因序列的重组质粒作为DNA疫苗的可能性。方法采用PCR方法获得HSV-2gD片段,构建含HSV—gD片段的重组质粒pcDNA3—gD。重组质粒作为DNA疫苗免疫小鼠,观察其对小鼠免疫效果及保护作用。结果重组质粒pcDNA3-gD免疫小鼠后可产生特异性抗体,可保护小鼠免受HSV-2的致死性攻击,存活率达75％。结论重组质粒pcDNA3-gD可诱导小鼠产生特异性免疫反应, 可以作为HSV的候选DNA疫苗。更多还原

【Abstract】 Objective To evaluate the protective efficacy of DNA vaccine expressing herpes simplex type 2 (HSV-2)full-length glycoprotein D (gD).Methods The whole encoding sequence of gD was amplified from HSV-2 genome by PCR,and then cloned into downstream of PCMV of pcD-NA3,and resulted in the HSV-2 DNA vaccince-pcDNA3-gD.pcDNA3-gD was inoculated to mice by bilateral intramuscular injection into the rear legs.Results The mice inoculated with pcDNA3-gD could produce specific antibodies and survive after the lethal challenge with HSV-2.The rate of survival rate was 75%.Conclusion pcDNA3-gD can induce specific immune response and may be a candidate DNA vaccine D.更多还原