【作者】 徐水祥； 周淡宜； 徐飞鹤； 蒋有水；

【Author】 Xu Shui-xiang,Zhou Dan-yi,Xu Fei-he,et al (Institute of Health Food,Zhejiang Academy of Medical Sciences,Hangzhou,310013)

【机构】 浙江省医学科学院保健食品研究所； 浙江大学药学院； 杭州中得保健食品有限公司；

【摘要】 本文研究了甲鱼油中多不饱和脂肪酸的提取与纯化。采用乙酯化法提取甲鱼油中的多不饱和脂肪酸,通过正交实验确定了最佳提取条件:乙醇/甲鱼油比0．73:1,NaON用量3％(油重),反应温度65℃,反应时间 2h;采用尿素包合法纯化多不饱和脂肪酸,考察尿素用量、搅拌时间、包合次数对尿素包合率的影响,通过正交实验确定最佳纯化条件:尿素/乙醇饱和溶液:脂肪酸乙酯=15:1,搅拌时间3h,包合次数1次。实验结果表明,应用乙酯化法与尿素包合法可从甲鱼油中制得高纯度的多不饱和脂肪酸。更多还原

【Abstract】 The research on extracting and Purification PUFA from turtle oil was studied in this article.The PUFA was extracted by interesterification and the best extracting conditions were:the rate of ethanol to turle oil 0.73:1,sodium hydroxide 3%(of oil weight),reaction temperature 65℃,reaction time 2h.The PUFA was purified by urea inclusion and the best purification conditions were: the rate of urea-ethanol saturation solution to fatty acid ethyl ester 15:1,mixing round time 3h,in-clusion time 1.The result showed that high purified PUFA was made from turtle oil by interesterification and urea inclusion.更多还原