【作者】 梅玲玲； 高雯洁； 朱敏； 潘军航； 张俊彦； 汪炜； 姜德霞； 张严峻；

【Author】 Mei Lingling~1 gao wenjie~2 zhu min~1 pan junhang~1 zhang junyan~1 wan hui~1 jiang dexia~1 zhang yanjun~1 1 The Center for Disease Prevention and Control of Zhejiang Province,Hangzhou 310009 2 The Center for Disease Prevention and Control of Jiaxing City,Jiajing 314000

【机构】 浙江省疾病预防控制中心； 嘉兴市疾病预防控制中心；

【摘要】 目的：掌握浙江省海、淡水鱼中香港海鸥型茵污染状况,为防止食源性香港海鸥型菌病的发生和流行提供科学依据；方法：用改良头孢哌酮麦康凯琼脂分离、API 20NE 鉴定香港海鸥型菌。K-B 纸片扩散法作耐药性测定。通用引物扩增16S rDNA 并与香港海鸥型菌 HKU1株开展同源性比较。结果：369份样品检出香港海鸥型茵18株,阳性率为4．88%,其中,鲤鱼检出率为25．00%, 草鱼的阳性率为10．26%,海水鱼以及鲫鱼、鲢鱼、扁鱼等淡水鱼未分离到本菌；分离的香港海鸥型茵16s rDNA 序列与 HKU1株仅相差1～2个碱基,同源性在99．6%～100．0%之间；18株菌对头孢拉定、万古霉素、甲硝唑、克林霉素、头孢哌酮完全耐药,对红霉素、亚胺培南、克拉霉素、氨曲南、庆大霉素、丁胺卡那霉素、三甲氧苄氨嘧啶、四环素、氯霉素、多粘菌素 B、环丙沙星完全敏感, 对头孢噻吩、胺苄西林、头孢曲松的耐药性分别为83．33%、61．11%和5．56%。结论：浙江省存在香港海鸥型菌污染源,建议相关监督部门应加大这类产品的管理力度,防止香港海鸥型菌病在我省的发生和传播。更多还原

【Abstract】 Aim To find out the practical situation of Laribacter hongkongensis contamination in fresh water and sea fishes in Zhejiang Province so as to provide scientific base for the prevention of Laribacter hongkongensis occurrence and prevalence caused by food sources.Method Laribacter hongkongensis was isolated by improved Mac-Lonkey Agar(with 32 μg cefoperazone/ml)agar,and identified by API 20NE.Drug tolerance was tested by K-B paper sheet diffusion method.16S rDNA were amplified by general primer and homologous comparison with Laribacter hongkongensis strain HKU1 was carried out.Result 18 strains of Laribacter hongkongensis were isolated from an examination on 369 samples,the total contamination rate was 4.88%.However,the contamination rates detected in carp was 25.00%,grass carp 10.26%,and not found in sea fish and other fresh water fishes such as crucian,chub and pomfret.The sequence of 16S rDNA isolated from Laribacter hongkongensis was only 1～2 base pair difference by comparing with the HKU1 strain,a homology as high as 99.6%～100.0%.Drug tolerant ability test showed that 18 strains isolated in the present experiment are completely tolerant to ceftazidime、 vancomycin、metrorddazole、clindamycin、cefuroxime,but completely sensitive to erythromycin、 imipenem、clarithromycin、azetreonam、gentamicin、amikacin、Trimethoprim、tetraciline,chloromycetin, polymyxin B and ciprofloxacin;and its tolerant ability to cephalothin、ampicillin and ceftriaxone are 83.33%、61.11% and 5.56%、respectively.Conclnsion There is contamination source of Laribacter hongkongensis in Zhejiang Province,related monitoring departments should increase supervising strength on this category of food to prevent the occurrence and prevalence ofLaribacter hongkongensis in Zhejiang province.更多还原