【作者】 钱红兰； 胡倩； 胡旭东； 陈怡； 孙岚； 梁彬； 叶海格；

【Author】 Qian Honglan HUqian HU Xudong

【机构】 浙江省温州医学院附属第一医院血液内科；

【摘要】 目的:探讨槐耳浸膏对骨髓瘤细胞株 PRMI8226细胞增殖和凋亡的影响。方法:体外培养 PRMI8226细胞与1.5mg/ml、3mg/ml、5mg/ml 的不同浓度槐耳浸膏共孵育,分别在24h、36h、和 48h 时用 CCK-8法检测细胞增殖抑制率,并用流式细胞术(FCM)检测早期细胞凋亡情况。结果:槐耳浸膏可抑制 PRMI8226细胞细胞的增殖,不同浓度的槐耳浸膏对多发性骨髓瘤细胞增殖的抑制效果不同,以5mg/ml 时抑制增殖效果最好。且浓度为5.0mg/ml 的金克诱导多发性骨髓瘤细胞48小时抑制率达到高峰84%。FCM 检测表明槐耳浸膏可诱导 PRMI8226细胞早期凋亡,用5.0mg/ml 槐耳浸膏处理48h 后,可引起25.9%的PRMI8226细胞凋亡。这两种作用均随槐耳浸膏浓度和作用时间的延长而增强。结论:槐耳浸膏可在体外抑制 PRMI8226细胞增殖,并促进其凋亡。更多还原

【Abstract】 Objective:To investigate the effects of PS-T on proliferation and apoptosis of Myeloma cell line PRMI8226 in vitro.Methods:PRMI8226 cells were cultured with different concentrations of PS-T (1.5,3.0 and 5.0mg/ml).After 24,36 and 48 hours,the inhibition of proliferation was measured by CCK-8 assay,and cell apoptosis as evaluated by flow cytometry.Results:PS-T inhibited the growth of PRMI8226 cells in concentration and time-dependent manner.Flow cytometry analysis showed that PS-T induced apoptosis of PRMI8226 cells,and the percentage ofapoptotic cells increased as the concentration increased and time elapsed.Conclusion:PS-T inhibit proliferation of PRMI8226 cells and promote its apoptosis.更多还原