【作者】 龚朝辉； 乐燕萍； 金勇丰； 陈健； 张耀洲；

【Author】 GONG Zhao-Hui LE Yan-Ping JIN Yong-Feng CHEN Jian ZHANG Yao-Zhou (School of Medicine, Ningbo University, Ningbo 315211; College of Life Sciences, Hangzhou 310058; College of Life Sciences, Hangzhou 310018)

【机构】 宁波大学医学院； 浙江大学生命科学学院； 浙江理工大学生命科学学院；

【摘要】 Bombyxin是一类在家蚕脑组织中合成的胰岛素相关多肽．本研究家蚕幼虫中克隆了一个bombyxin基因家族的新成员-G基因．该基因的开放阅读框(ORF)编码一个前体分子, 由四个结构域构成:信号肽,B链,C肽和A链,并与已知的bombyxin基因有99％的序列同源性．Bombyxin蛋白的二级结构预测表明α-螺旋占45．6％,延伸线占10．0％,β-转角占4．4％, 无规卷曲占40．0％．而新克隆的G基因缡码的蛋白质在昆虫体内的天然功能有待进一步研究。更多还原

【Abstract】 Bombyxin is an insulin-related peptide synthesized in the brain of the silkworm Bombyx mari. Here we cloned a new member of bombyxin gene family (named G gene) from the silkworm larvae. The open reading frame (ORF) of G gene encodes a precursor molecule which consists of four domains in the order of signal peptide, B chain, C-peptide and A chain and shares 99% sequence identity with bombyxin previously characterized. The result of protein prediction demonstrates that there are 45. 6% of a-helix, 10. 0% of extend strand, 4. 4% of β-turn and 40. 0% of random coil in the secondary structures. Whereas the natural function in insect biology of this newly cloned bombyxin G protein need to be further investigated.更多还原