【作者】 胡京红； 王淑华； 卢咏才；

【Author】 HU Jing-hong WANG Shu-hua LU Yong-cai(Beijing University of Tradition Chinese Medicine,Beijing 100029)

【机构】 北京中医药大学；

【摘要】 目的:探讨淋巴细胞条件培养液和高脂血清影响平滑肌细胞(SMC)生物学行为在动脉粥样硬化发生发展过程中的意义。方法:用培养的人主动脉SMC吞噬中性红的方法,观察正常SMC经淋巴细胞条件培养液诱导及高脂血清刺激等作用后,细胞吞噬功能的改变。结果:高脂、激活淋巴细胞条件培养液及高脂血清+淋巴细胞条件培养液均可使SMC出现吞噬功能,其中以淋巴细胞条件培养液的影响最为显著。结论:高脂和淋巴细胞释放的某些淋巴因子能触发SMC的生物学行为改变,如出现异常HLA-DR抗原表达,使其获得吞噬功能,吞噬脂质形成泡沫细胞,从而在动脉粥样硬化的形成中起重要作用。更多还原

【Abstract】 We cultured the aortic smooth muscle cell(SMC)with limphocyte conditioned medium(LCM)and hyperlipemic serum(HLS),to demonstrated the phagocytic faction of SMC with neutralred ingestion method in vitro.The results showed that LCM,HLS and LCM+HLS all can induce the phagocyotic activity of SMC was particularly active in the LCM culture.It suggests that LCM may contain certein certein lymphocyte factors which trigger the change of SMCin biologic behavior,so as to gain the ability of phagocyte which englove lipid and become a foam cell and play an important role in the pathogenesis of atherosclerosis.更多还原