【作者】 林刃舆； 陈建福；

【Author】 Lin Ren Yu* Chi Fang Lu Chen Jian Fu. (Department of Otorhinolaryngology,the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000,China)

【机构】 温州医学院附属第一医院耳鼻喉科；

【摘要】 <正>目的探讨 EGFR 和磷酸化酪氨酸在中耳继发性胆脂瘤的表达情况,分析酪氨酸蛋白激酶途径在胆脂瘤上皮连续增殖演变过程中的作用。方法应用免疫组化 SP 染色方法和计算机图像分析系统,连续观察10例具有典型鼓膜后皱襞处穿孔的中耳继发性胆脂瘤患者的不同部位(胆脂瘤上皮、鼓膜穿孔部位邻近皮肤、耳道深部正常皮肤)中 EGFR 和磷酸化酪氨酸的表达情况。更多还原

【Abstract】 Objective To explore the expressions of EGFR and phosphotyrosine in acquired middle ear cholesteatoma,to analyze the signal transduction pathway of protein tyrosine kinases playing the possible role in proliferation ability of cholesteatoma epidermis.Methods The specimens from 10 cases acquired middle ear cholesteatoma patients having typical perforation in the posterior fold(the cholesteatoma tis- sue、the adjacent skin around perforation and the normal deep meatal skin)were examined by immunohis- tochemical S-P method and computer image analysis.Results From the normal deep meatal skin to the cholesteatoma epidermis,EGFR and phosphotyrosine were continuously ascending expressions.The expressions of EGFR and phosphotyrosine in all epithelial layers of cholesteatoma were abundant stained, especially in the basal and the spinous layers;the basal and spinous layers were moderate stained in the adjacent skin around perforation;and only the basal layer were slightly stained in the normal deep meatal skin.There was positive correlation between the two groups,the total coefficient of correlation was 0.989 (P＜0.01 ).Conclusions The protein tyrosine kinases(PTK)pathway play pivotal role during the dif- ferentiation and the hyperproliferation of cholesteatoma.The overexpressions of EGFR and phosphoty- rosine in the cholesteatoma tissue indicated that the cholesteatoma epidermis possessed a strongly hyper- proliferative power,and the adjacent skin around perforation had a relatively hyperproliferative ability.更多还原