【作者】 朱敏； 章强强； 李莉； 王家俊； 陈玉；

【Author】 Min Zhu, Qiangqiang Zhang, Xiasheng Wang, Jiajun Wang and Yu Chen Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China

【机构】 复旦大学附属华山医院皮肤科；

【摘要】 目的:筛选有效抑制马拉色菌的中草药及其单体。方法:参照M27-A方案中的酵母菌微量稀释法进行药敏试验。结果:选择23种中草药及14种单体对马拉色菌进行体外抑菌初筛试验,结果显示23种中草药中,香茅、蛇床子对马拉色菌有较强的抑制作用(MIC31．25～62．5mg／L);14种中草药单体中,苦参碱、氧化苦参碱、柠檬醛、丁香酚有强的抗马拉色菌作用(MIC0．98～7．81mg／L)。5 味中草药混合液及5种单体混合液的MIC值均不低于其单味药的MIC值。在此基础上,进一步选择苦参碱、柠檬醛、丁香酚3种中草药单体对7种马拉色菌参考菌株及5种临床分离株进行体外抑菌、杀菌试验,并在培养基中加入10％人血清,观察高蛋白环境下3种单体的抑菌情况,计算其抑菌活性降低分数。结果显示,苦参碱对M.globosa、M.slooffiae、M.furfur具强抑菌作用(MIC0．39～0．78 mg/L), 柠檬醛对M.globosa、M.slooffiae有强抑菌作用(MIC0．195～0．78 mg／L),丁香酚对M.globosa、 M.furfur抑菌作用最强(MIC0．195～0．78 mg／L)。其中M.globosa对3种单体均最敏感,尤其是对柠檬醛、丁香酚,其MTC值为0．195mg／L。苦参碱对M.restricta的MFC值及丁香酚对M.globosa的MFC 值均较其MIC值高几个浓度梯度,其余的MFC与MIC值相同或高1个浓度梯度。3种单体中,柠檬醛与蛋白亲和力最高,抑菌活性降低分数为1．3。结论:中药或中药的有效成分对马拉色菌的抑制无相互协同作用。M27-A方案中的酵母菌微量稀释法同样适用于中草药对马拉色菌的体外药敏试验。更多还原

【Abstract】 Objective: To get Chinese herbs and components which inhibit Genus Malassezia effectively. Method: The susceptibilities of Malassezia in vitro were determined by M27-A microdilution method . Results: Cymbopogon citratus, Cnidium monnieri have strong and- Malassezia activity (MIC31.25-62.5mg/L) from 23 kinds of Chinese herbs. Matrine, oxymatrine, citral, eugenol have stronger anti-Malassezia activity from 14 kinds of Chinese herbal components (MIC0.98-7.81mg/L) . Matrine ,citral and eugenol were selected in the further fungistatic and fungicidal activity testings.It has shown that M.globosa was the species most susceptible to 3 components as mentioned above,especially to citral and eugenol(MIC 0.195mg/L ).The MFCs of matrine and eugenol were several gradients higher for M.restricta and M.globosa , respectively, while others were equivalent or 1 gradient apart between MFC and MIC. To predict the anti-Malassezia activity of the 3 components in infected skin tissues,we investigated the effect of serum as a keratinic substance on the in vitro activity of them. 10% human serum-containing Malassezia broth medium was used. The genomic MIC of 3 components to Malassezia species referance strains were taken. The reduced activity of citral was 1.3 which was lower than other 2 components and had a highest affinity to keratin. Conclusions: No synergistic inhibitive action to Malassezia were observed among Chinese herbs or their effective components. Microdilution method from M27-A was also available for the susceptibility of Malassezia in vitro to Chinese herb medicine.更多还原