【作者】 顾寿永； 张忠彬； 曹多志； 万俊香； 高晓玲； 金锡鹏； 夏昭林；

【Author】 GU Shouyong ZHANG Zhongbin, CAO Duozhi, WAN Junxiang, GAO Xiaoling, JIN Xipeng,XIA Zhaolin .Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China

【机构】 复旦大学公共卫生学院劳动卫生教研室； 马鞍山钢铁公司医院劳动卫生研究所； 上海市第一人民医院；

【摘要】 [目的]探讨探讨细胞色素P450 1A1利2D6基因多态性与慢性苯中毒易感性的关系。[方法]采用病例一对照研究,选择152名苯中毒工人为病例组,152名接触苯而无中毒表现的工人为对照组。采用PCR-RFLP技术检测CYP1A1基因3’端非编码区Msp I和CYP2D6第1外显子c.188位点、g.212位点多态性。[结果]携带CYP1A1 Msp□T/T基因型的个体发生苯中毒的危险性是携带有CYP1A1Msp□T/C或C/C基因型的个体的1.32倍(95%CI 1.05-1.65,P=0.02):在不吸烟的人群中,携带CYP1A1Msp□T/T基因型的个体发生苯中毒的风险性是携带T/C或C/C基因型的个体的1.56倍(95%CI:1.15-2.12,P=0.003);携带有CYP2D6 c.188 C/C或C/T基因型个体发生苯中毒的危险性是携带有CYP2D6c.188T/T基因型的个体的1.23倍(95%CI:1.05-1.42,P=0.01),在不吸烟的人群中,携带CYP2D6c.188 C/C或C/T基因型个体发生苯中毒的危险性是携带有CYP2D6 c.188T/T基因型的个体的1.23倍(95%CI:1.04-1.47,P=0.01)。未发现研究对象的CYP2D6g.212位点存在多态性。[结论]携带CYP1A1 Msp□T/T基因型、CYP2D6 C.188 C/C和C/T基因型个体对苯中毒可能易感更多还原

【Abstract】 [Objective] To explore the association of genetic polymorphisms of CYP 1A1 and CYP2D6 with risks of chronic benzene poisoning (BP). [Methods] A case-control study was conducted. 152 BP patients and 152 workers occupationally exposed to benzene without poisoning manifestations were involved. Polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) technology were used to detect the single nucleotide polymorphisms (SNPs)of Msp I in the non-coding region of CYP 1A1 gene and c. 188,g.212 position in the first extron of CYP2D6 gene. [Results] The individuals with CYP1A1 Msp I T/T genotype had a 1.32-fold (95%CI: 1.05-1.65, P=0.02) increased risk of BP compared with those carrying T/C and C/C genotypes. In no-smoking population, there was a 1.56-fold (95%CI: 1.15-2.12, P=0.003) increased risk of BP for subjects carrying CYP1A1 Msp I T/T genotype compared with those carrying T/C and C/C genotypes. The individuals carrying CYP2D6c.188 C/C or C/T genotype had a 1.23-fold (95%CI: 1.05-1.42,P=0.01) increased risk compared with those carrying T/T genotypes. In no-smoking population, there was a 1.23-fold (95%CI: 1.04-1.47, P=0.01) increased risk of BP for subjects carrying CYP2D6c.188 C/C or C/T genotypes compared with those carrying T/T genotype. The single nucleotide polymorphism of g.212 position in the first extron of CYP2D6 gene has not been validated. [Conclusion] It was suggested that the individuals with CYP2D6 c.188 C/C, CYP2D6 c.188 C/T and CYP1A1 Msp /T/T genotypes tended to be more susceptible to benzene toxicity.更多还原