【作者】 安丽萍； 吕刚； 李贺； 杜培革；

【Author】 AN Li-Ping LU Gang LI He DU Pei-Ge (Beihua University,Jilin132013, China)

【机构】 北华大学；

【摘要】 将纯化的大鼠TPPI分别与NMB、强啡肽(dynorphin)等小分子肽及基质Ala-Ala—Phe-MCA混合,在相同的条件下,不同时间观察TPPI对上述肽的水解作用。通过高效液相层析及飞行质谱仪,在体外分析了大鼠TPP I对NMB及强啡肽等几种物质的水解作用。结果表明,大鼠TPP I能在16 h内完全水解NMB,与水解Ala-Ala-Phe-MCA的速度相同,并释放三肽GNL,而在24 h内未消化强啡肽等几种物质。TPP I是降解NMB的关键水解酶。NMB是TPP I的天然基质。更多还原

【Abstract】 The mixture of rat TPP I and NMB and other neuropeptides and Ala-Ala-Phe-MCA is made respectively on the same condition,observed the reaction at the different times. That is assayed by high performance liquid chromatography ( HPLC) and time-of-flight mass spectrometry(TOF-MS) in vitro. Result It showed NMB was cleaved to the same degree as Ala-Ala-Phe-MCA by the rat TPP I and released GNL within 16 hours,but dynorohin and other materials couldn’ t been digested within 24 hours .Conclusion TPP-I is the predominant proteolytic enzyme responsible for the intracellular degradation of neuromedin B. NMB is a good natural substrate of TPP I.更多还原