【作者】 张忠民； 陈建庭； 金大地； 武大林； 陈祥鋆；

【Author】 Zhongmin Zhang, Jianting Chen, Dadi Jin, et al.Department of orthopaedics and spinal surgery, Nan Fang ospital,first military medical university, Guangzhou 510515

【机构】 第一军医大学南方医院骨科；

【摘要】 目的:研究血小板衍生生长因子(PDGF)-AA对破骨细胞功能的影响.方法:利用免疫电镜技术证明PDGFα受体在破骨细胞膜得以表达;酶动力学测定培养基中酸性磷酸酶与抗酒五酸酸性磷酸酶活性;通过荧光探针在共聚焦显微镜下观察破骨细胞内氢离子随PDGF-AA浓度的变化情况;利用甲苯胺蓝对骨吸收陷窝染色井在图像分析仪下测定其面积和数目.结果:破骨细胞膜有胶体金颗粒沉着,抗酒石酸酸性磷酸酶活性的无显著变化(P>0.05),细胞内氢离子显著增加,但PDGR-AA不能促进其释放;骨吸收陷窝的面积与数目均无显著变化.结论:PDGF-AA虽然能够促进氢离子在细胞内产量增加,但由于不能显著改变抗酒石酸酸性磷酸酶活性,因而不能直接促进破骨细胞的骨吸收功能.更多还原

【Abstract】 Objective:to study the effects of platelet - derived growth factor ( PDGF)-AA on the osteoclasts. Methods: the PDGF receptor a (PDGFR-a) was detected on the osteoclastic membrane with immuno-electronmicroscopy.The acid phosphaiase (ACP) and tartrate - resistant acid phosphatase (TRAP) activities were measured kineticly and the introcellular hydrogen ions were calculated on the confocal laser scanning microscope (CLSM) stained with SNAFL.Stained by toludine blue, the area and number of the resorption pits were determined with the Leica Quantimet 500 system. Results: the colloidal gold precipitated on the cellular membrane, and the TRAP activity had no difference (p>0.05) . The hydrogen ions rose significantly but their release could not be promoted by PDGF-AA. The changes of the resorption pits were not significant. Conclusions: the PDGF-AA could promote hydrogen ions yielding but not stimulate osteoclastic bone resorption due to the TRAP changes of TRAP activities having no significance.更多还原