【作者】 杨宗保； 严洁； 邹晓平； 易受乡； 常小荣； 林亚萍； 唐森；

【Author】 Zong-Bao Yang, Jie Yan, Xiao-Ping Zou, Shou-Xiang Yi, Xiao-Rong Chang, Ya-Ping Lin, Sen Tang, Department of Acupuncture and Moxibustion, HuNan College of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China

【机构】 湖南中医药大学针灸推拿学院；

【摘要】 目的:观察针刺胃经穴后的血清对胃溃疡大鼠胃黏膜细胞磷脂酶-Cγ-1(PLCγ-1)活性的影响．方法:大鼠60只随机分为溃疡血清组、胃经血清组、胆经血清组、胃经血清+PD153035 组和胆经血清+PD153035组,采用水浸束缚法制作胃溃疡模型,利用链霉蛋白酶消化法分离胃黏膜细胞,分别用表皮生长因子受体 (EGFR)抑制剂PD153035和血清孵育胃黏膜细胞,应用酶联免疫吸附(ELISA)法检测PLCγ-1 活性．结果:胃经血清组(13．0±7．5 nkat/g)大鼠胃黏膜细胞PLCγ-1活性与溃疡血清组(6．5±0．3 nkat/g)比较有显著性差异(P=0．01);胃经血清组大鼠胃黏膜细胞PLCγ-1活性与胆经血清组 (7．2±1．8 nkat／g)比较有显著性差异(P=0．02); 胃经血清组大鼠胃黏膜细胞PLCγ-1活性与胃经血清+PD153035组(7．4±2．7 nkat／g)比较有显著性差异(P=0．02)．结论:针刺胃经穴后的血清能刺激胃黏膜细胞中PLCγ-1的活化,并且存在经脉-脏腑的特异性联系．更多还原

【Abstract】 AIM: To observe the activity of phospholipase Cγ-1 (PLCγ-1) in gastric mucosal cells of rats with gastric ulcer after treatment with acupuncture serum. METHODS: Sixty rats were randomly divided into model serum (MS) group, gastric meridian serum (GMS) group, gallbladder meridian serum (GBMS) group, GMS plus PD153035 group and GBMS plus PD153035 group. Rat model of gastric ulcer was established by water immersion and restrained stress methods, and the gastric mucosal cells were separated by pronase digestion and then incubated by serum and inhibitor of epidermal growth factor receptor PD153035, respectively. The activity of PLCγ-1 in gastric mucosal cells was detected by enzyme linked immunosorbent assay (ELISA). RESULTS:In comparison with that in MS group (6.5±0.3 nkat/g), the acupuncture serum in GMS group had a tendency to improve the activity of PLCγ-1 (13.0±7.5 nkat/g) in gastric mucosal cells (P=0.01), and this tendency existed in GBMS group (7.2±1.8 nkat/g) but with no significant difference (P > 0.05). The activity of PLCγ-1 was also significantly higher in GMS group than that in GBMS group (72±1.8 nkat/g, P = 0.02) or GMS plus PD153035 group (7.4±2.7 nkat/g, P = 0.02).更多还原