【作者】 李瑞午； 张金铃； 郭莹； 李翠红；

【Author】 LI Rui-wu, ZHANG Jin-ling, GUO Ying, LI Cui-hong (China Academy of TCM, Institute of Acupuncture and Moxibustion, Beijing 100700, China)

【机构】 中国中医研究院针灸研究所；

【摘要】 目的:观察针刺大白鼠经穴后的血清对体外培养的大脑皮层细胞内钙离子浓度的影响,以探讨针灸作用机理中是否存在着体液因素。方法:取新生大鼠大脑皮层细胞进行原代培养,7-10 天后应用荧光分子探针Fluo-3AM染色,激光共焦扫描显微镜动态扫描观察加入正常血清和电针刺“百会”“足三里”“曲池”“三阴交”的血清后神经细胞内钙离子的浓度变化。结果:加入正常血清后,细胞内的钙离子浓度会有一定的升高,经过一段时间后升高的钙离子浓度会趋向平衡;而再加入针刺血清后,在一定程度上可以降低神经细胞内钙离子的浓度,结论:针刺腧穴后,除了神经的调节机制外,针刺血清中存在着某种活性物质可以明显地使大脑皮层细胞内钙离子浓度降低。此为针灸体液因素的存在提供了直接的证据。更多还原

【Abstract】 Objective To observe effect of serum of the rat after acupuncture on Ca2+ content of cultured nervous cells in vitro for exploring the role of humoral factors in acupuncture. Metbods Neurons of the cerebral cortex from the new born rats were taken and were cultured in a medium, 7-10 days later, fluorescein-molecular probe Fluo-3AM was used for staining of intracellular Ca2+. The intracellular Ca2+ levels in the nervous cells cultured with the serum of the normal rats or the rats given acupuncture at "Baihui" (GV 20), "Zusanli" (ST 36), "Quchi" (LI 11) and "Sanyinjiao" (SP 6) for 2 weeks were determined by using a laser confocal microscope. Results After addition of the serum of normal rats, the intracellular Ca2+ level increased and then gradually got stable, and when the acupuncture serum was added to the cultured medium, its level decreased to a certain extent. Conclusion Acupuncture serum can significantly decrease Ca2+ level in cultured cells of the cerebral cortex, which provides a direct evidence for involvement of humoral factors in the role of acupuncture.更多还原