【作者】 沈世林； 赵健雄； 朱玉真； 王学习； 徐静汶； 王敏；

【机构】 兰州军区总医院博士后工作站； 兰州大学中西医结合研究所；

【摘要】 目的观察扶正抑瘤颗粒(FYK)对小鼠肿瘤细胞凋亡相关基因P53、BCL-2蛋白表达的影响。方法将48只小鼠分成FYK大剂量组(D)、FYK小剂量组(X)、天仙胶囊阳性对照组(T)和荷瘤模型组(M),造模形成实体瘤后分别灌胃FYK、天仙胶囊、生理盐水18天,取瘤组织用流式细胞仪检测P53、BCL-2蛋白表达。结果使用FYK后,肿瘤细胞P53蛋白表达升高、BCL-2蛋白表达下降,与M组相比较有显著性差异(P<0．01)。结论 FYK具有促进肿瘤细胞P53表达和抑制BCL-2表达作用。更多还原

【Abstract】 Objective To observe FYK on the Protein expression of genes related to apoptosis P53 and BCL-2 in tumor cells of H22 solid tumor mice. Methods 48 mice were divided into four groups: high dose group FYK (D) ,low dose group FYK (X) .control group(T) and model group(M). All nicer were inoculating H22 tumor strain to build a model of solid tumor. Four groups were administrated respectively by gastrogavage with FYK, TianXian capsule and NS for 18 days. All leth day, Killed mice, Cut and get tumor tissue, detected Protein expression of P53 and BCL-2 applying technical of flow cytometry(FCM). Results The Protein expression of P53 was high and Protein expression of BCL-2 was low by treatment of FYK, which were significant compared with model group(P<0.01). Conclusion FYK could promote expression of P53 and inhibited expression of BCL-2 in tumor cells.更多还原