【作者】 张永和； 黄晓巍； 欧喜燕； 邓毅峰； 周鸣；

【机构】 长春中医药大学中西医结合基础学科附属医院新药中心；

【摘要】 目的观察鹿茸提取物对心肌缺血动物模型心肌损伤急性期后血管内皮生长因子 (VEGF)变化的的影响,并探讨其对心肌细胞保护作用的机制。方法采取结扎大鼠冠状动脉左前降支的方法复制心肌缺血模型,在给药21天后运用免疫组织化学SABC法测定其心肌细胞血管内皮生长因子的表达。结果图象分析系统统计结果显示:模型组大鼠心肌中VEGF 含量明显高于空白对照组(P<0．05),证明急性心肌缺血时,机体代偿性地增高VEGF含量以促进侧枝循环的建立而改善心肌缺血的状况。与模型组比较,鹿茸高剂量组和中剂量组均能不同程度地增加心肌缺血区域的VEGF表达(P<0.00l,P<0．01)。结论鹿茸可通过提高 VEGF的含量以促进缺血心肌中侧枝循环的建立而改善其缺血的情况,起到对缺血心肌的保护作用。更多还原

【Abstract】 Objective To observe the effect of Cornu Cervi on the change of VEGF after the acute damage of myocardium in animal model of myocardial ischemia, and inquired into the mechanism of the protective effect on myocardium cell. Methods We adopted the myocardial ischemia rat model by ligaturing their front-fall branch of left coronary attery, and tested the convey of VEGF in myocardium cell by means of immunohistochemistry 21 days after having given Cornu Cervi. Results The statistics result of analytical picture system showed: the value of VEGF of model control was obviously higher than that of blank control group (P<0.05). From it we can prove that, during the period of acute myocardial ischemia, the level of VEGF could increase vicariously to promote building of collateral circulation in order to improve the condition of myocardial ischemia. The convey of VEGF of high dose group and middle dose group was higher than that of model control group in the area of myocardial ischemia in varying degrees (P<0.001, P<0.01). Conclusion Cornu Cervi could promote building of collateral circulation in ischemia myocardium to improve the condition of ischemia with raising the value of VEGF, so that it could protect the ischemia myocardium.更多还原