【作者】 邓常青； 唐映红； 梁燕； 杨静； 张淑萍；

【机构】 湖南中医药大学病理生理实验室；

【摘要】 目的探讨FeCl3诱导的大鼠动脉血栓形成模型血浆TXA2、6-keto-PGF1α含量及抗凝、纤溶活性的变化。方法以FeCl3外敷诱导大鼠左侧颈总动脉血栓形成,测定血浆TXA2、 6-keco-PGF1α含量和t-PA、PAI、PLG、AT-Ⅲ和PC活性。结果 35％FeCl3可诱导大鼠左侧闭塞性血栓形成,且该模型血浆TXB2含量升高(P<0．05),6-keto-PGF1α含量降低(P<0．05); 血浆t-PA和PLG活性降低(P<0．05),PAI活性无显著性变化(P>0．05),t-PA／PAI比值降低 (P<0．05);血浆AT-Ⅲ活性降低(P<0．01),PC活性轻微降低(0．10>P>0．05)。抵克利得可抑制FeCl3诱导的血栓形成,但对TXB2和6-keto-PGF1α无显著影响,表明其抗栓作用不是通过抑制血小板TXA2生成而得的。抵克利得还可升高血浆t-PA活性,可能系该药通过抑制血栓形成,减少了血中t-PA消耗所致。而抵克利得对AT-Ⅲ和PC活性无显著影响。抗凝血药低分子肝素(LMWH)也可抑制血栓形成,该药对TXB2和6-keto-PGF1α无显著影响。 LMWH可升高血浆t-PA活性和t-PA／PAI比值,可能与其促进血管内皮细胞释放t-PA有关。 LMWH还可使该模型血浆AF-Ⅲ活性降低,这是由于LMWH与AT-Ⅲ结合而发挥其抗凝作用,从而使血浆中AF-Ⅲ消耗增多所致。结论 FeCl3可诱导大鼠闭塞性血栓形成,该模型与血小板活化和凝血系统激活有关,同时该模型存在抗凝蛋白活性降低和纤溶活性降低的病理改变。更多还原

【Abstract】 AIM To investigate changes of TXA2 and PGI2 content and anticoagulative and fibrinolytic activity in the plasma in a rat model of topical FeCl3-induced carotid artery thrombosis. Method Thrombi was induced by topical ferric chloride to left carotid artery .The content of TXB2 and PGI2 .activity of t-PA,PAI,PLG, AT-Ⅲ and PC in the plasma in the model of thrombosis were determined. Results Occlusive thrombi was induced by applying 35% FeCl3 to left carotid artery .The contents of TXB2 were increased markedly (P0.05), the contents of 6-keto-PGF1α were lower remarkably in the rat plasma (P<0.05). Activity of t-PA, PLG and AT-Ⅲ were lower in the plasma (P<0.05, P<0.01), activity of PAI had no changes (PX).05) ,t-PA/PAI ration reduced (P<0.05). Activity of PC slight dropped (0.10>PX).05). Ticlopidine could inhibit thrombosis induced by ferric chloride, but it did not influence TXB2 and 6-keto-PGF1α content and activity of AT-Ⅲ and PC in the plasma. Ticlopidine could enhance activity of t-PA in the plasma, it may be related to decrease of t-PA comsuption by inhibiting thrombosis. Antithrombosis action of ticlopidine may not be concerned to inhibiting production of TXA2 of platelet . LMWH could inhibit thrombosis also, but it did not influence TXA2 and 6-keto-PGF1α contents in the plasma. LMWH could enhance activity of t-PA and ratio of t-PA/PAI, it may be related to promoting release of t-PA in vascular endothelial cells. LMWH could reduce activity of AT-Ⅲ. It may be concerned with combination of LMWH and AT-Ⅲ result in increase of AT-Ⅲ comsuption . Conclusion Ferric chloride may induce occlusive thrombosis in rat. Thrombosis may be assiated with activation of platelet and blood coagulation system, lower of anticoggulation and fibrinolytic activity.更多还原