【作者】 董竞成； 董晓辉；

【机构】 复旦大学附属华山医院中西医结合肺和特应性疾病研究室；

【摘要】 目的:黄芪注射液与IL-2增强树突细胞的抗肿瘤转移作用的比较。方法:制备小鼠骨髓来源的树突细胞,用转移性Lewis肺癌特异性多肽Mutl预激树突细胞并联合黄芪注射液或者IL-2治疗转移性肺癌小鼠,通过FACS分析其脾细胞内T淋巴细胞比例的变化,用Elisa法检测荷瘤小鼠血清内IL-2、IL-4浓度的变化,间接提示Th1/Th2比例的变化。同时免疫正常小鼠,观察宿主对随后肿瘤细胞攻击的保护作用。结果:肿瘤抗原多肽致敏的树突细胞与黄芪注射液或者IL-2联合治疗后,肺癌结节减少,小鼠脾细胞内CD4~+T和CD8~+T细胞明显比例升高,血清IL-2/IL-4比例也明显升高,两组结果相似。在观察时间内经IL-2和DCs联合免疫的小鼠成瘤率低于对照组和单用DCs组。结论:黄芪注射液和IL-2都能增强树突细胞的抗肿瘤转移作用,有效的促进荷瘤宿主的免疫应答,具有显著的体内抑制肺癌转移的效果。对正常动物免疫保护作用更加明显。更多还原

【Abstract】 Objective: Dendritic-cell-based vaccines were established.We invetigated the antitumor effects and the possible mechanisms of tumor antigen peptide-pulsed dendritic cells in combination with Astragalus or Interleukin-2.We also compared with the effects of them. Methods:High purity DCs were obtained from 9-day culture of murine bone marrow cells. Mutl is (?)umor antigen peptide of Lewis lung cancer.DCs were incubated with Mut1,then vaccines were .established.Lung cancer were established on the mice.The tumor-bearing mice were treated and the survival period of the mice were observed.Treatment groups were divided into PBS,DC-Mut1, DC-Mut1 with Interleukin-2(DC-Mut1+IL-2),and DC-Mut1 with Astragalus(DC-Mut1+AG).The phenotypes of splenocytes of these treated mice were detected by FACS.Interleukin-2 and interleukin-4 in the serum were measured by cytokine-specific ELISA.Normal mice developed a potent immune protection against the challenge with tumor cells. The treatment groups were same as the previous groups.Results: The DC-Mut1+AG group exhibited a demonstrated antitumor effect with the majority of tumors undergoing regression. The effects were same as those of DC-Mut1+IL-2 group.The proportion of the CD3~+T, CD4~+T and CD8~+T cells of DC-Mutl+AG and DC-Mut1+IL-2 group increased compared with those of PBSgroup(P<0.05).The IL-2 levels of DC-Mutl +AG and DC-Mut1+IL-2 were both higher than those of PBS group(P<0.05). The IL-4 levels of DC-Mutl+AG group and DC-Mut1+IL-2 group were both lower than those of the PBS group(P<0.05). Upon the challenge with LLC cells,all the mice treated with AG and PBS developed progressively tumors in the period of 21 days.DC-Mut1+AG group and DC-Mut1+IL-2 group remained disease-free.In contrast,4 of all the mice(6) treated with DC-Mut1 developed tumors .Conclusion:Both AG and IL-2 could augment the efficacy of tumor antigen-pulsed DC in the intrduction of immune responses and could inhibit tumor spontaneous pulmonary metastasis.The effect of AG were almost same as Interleukin-2 on this point.更多还原