【作者】 董晓辉； 董竞成；

【机构】 复旦大学附属华山医院中西医结合肺和特应性疾病研究室；

【摘要】 目的:研究黄芪注射液联合(?)HC类限制性肿瘤抗原多肽Mutl致敏的树突细胞(DCs)对肺癌小鼠的治疗作用及其免疫学原理。方法:制备小鼠骨髓来源的树突细胞,用转移性Lewis肺癌特异性多肽Mutl预激DCs并联合黄芪注射液治疗肺癌小鼠,通过FACS分析其脾细胞内T淋巴细胞比例的变化,用Elisa法检测荷瘤小鼠血清内IL-2、IL-4浓度的变化,间接提示Th1/Th2比例的变化。同时免疫正常小鼠,观察宿主对随后肿瘤细胞攻击的保护作用。结果:肿瘤抗原多肽致敏的DCs与黄芪注射液联合治疗后,比单用DCs更有效的治疗转移性肺癌,小鼠脾细胞内CD4~+T和CD8~+T细胞明显比例升高,联合治疗组的IL-2/IL-4比例也明显升高。在观察时间内经黄芪注射液和DCs联合免疫的小鼠成瘤率低于对照组和单用DCs组。结论:以肿瘤抗原多肽致敏的DCs与黄芪注射液联合治疗能更有效的促进荷瘤宿主的免疫应答,具有显著的体内抑制肺癌转移的效果。对正常动物免疫保护作用更加明显。更多还原

【Abstract】 Objective: Dendritic-cell-based Vaccines were established.We invetigated the antitumor effects and the possible mechanisms of tumor antigen peptide-pulsed dendritic cells in combination with Astragalus.Methods:High purity DCs were obtained from 9-day culture of m(?) cancer.DCs were incubated with Mutl,then vaccines were established.Lung cancer were established on the mice.The tum0r-bearing mice were treated and the survival period of the mice Were observed.Treatment groups were divided into PBS,DC-Mutl,DC-Mutl with Astragalus(DC-Mutl+AG).The phenotypes of splenocytes of these treated mice were detected by FACS.Interleukin-2 and interleukin-4 in the serum were measured by cytokine-specific ELISA.Normal mice developed a potent immune protection against the challenge with tumor cells.The treatment groups were same as the previous groups.Results:The DC-Mutl+AG group exhibited a demonstrated antitumor effect with the majority of tumors undergoing regression. The proportion of the CD4~+T and CD8~+T cells of DC-Mut1+AG group increased compared with that of DC-Mutl group(P<0.05).The IL-2 levels of DC-Mut1+AG group were higher than those of DC-Mut1 group. The IL-4 levels of DC-Mut1+AG group were lower than those of the PBS group. Upon the challenge with LLC cells, DC-Mutl+AG group remained disease-free.In contrast,4 of all the mice(6) treated with DC-Mutl developed tumors.Conclusion: DC-Mut1+AG could mediate profound antitumor effects in the mice and significantly inhibit tumor metastasis,and demonstrate a specific immune protection.更多还原