【作者】 邓思珊； 阙慧卿； 张韬； 林绥； 夏志林；

【Author】 DENG Si-shan, QUE Hui-qing, ZHANG Tao, LIN Sui, XIA Zhi-lin (Fujian Institute of Medical Sciences, Fuzhou,350001)

【机构】 福建省医学科学研究所；

【摘要】 目的采用HPLC法建立雷公藤药材含量测定方法。方法药材经甲醇回流提取,经中性氧化铝吸附,三氯甲烷洗脱,蒸干后甲醇定容,色谱柱采用Hypersil BDS（250×4.0mm,5μm）,流动相为乙腈-水（23-77）（用于雷公藤内酯醇）,乙腈-甲醇-水（60-20-20）（用于雷公藤内酯甲）,检测波长:220nm（雷公藤内酯醇）,210nm（雷公藤内酯甲）。结果雷公藤内酯醇与雷公藤内酯甲在5-100μm^-1（r=0.9998,r=0.9986）浓度范围内线性关系良好,平均回收率为99.1%,97.2%（n=9）,RSD<2%。结论本法方法简便,灵敏度高,重现性好。更多还原

【Abstract】 objective To establish a HPLC method for the determination of Triptolide and Wilforlide in Tripterygium Wilfordil Hook.fo Method The analytical column was Hypersil BDS（250 × 4.0mm,5μm）, The mobile phase was acetonitrile-water（23-77）（Triptolide）and acetonitrile-methanol-water（60-20-20）（Wilforlide）, UV detection was carried out at 220nm and 210nm,The flow rate was 1.0mL.min^-1。Results Triptolide and Wilforlide calibration curves were linear within the range of5-100μg mL^-1（r=0.9998 and 0.9986）, The average recovery was 99.1% and 97.2%, RSD 〈2%。Conclusion The HPLC method was sensitive and accurate for determining Triptolide and Wilforlide in Tripterygium Wilfordil Hook.f。更多还原