【作者】 魏庆宇； 李刚； 柳春； 叶晶； 张萍；

【Author】 Wei Qingyu, ye Jing, Zhang Ping,et al .Department of Allergy Diagnosis, No .202 Hospital of PLA shenyang 110003

【机构】 沈阳军区202医院变态反应科； 辽宁中医学院；

【摘要】 目的:探讨细胞间粘附分子(ICAM-1)和白细胞介素-5(IL-5)在哮喘发病过程中的相互关系及加味玉屏风散对它们的影响。方法:采用卵蛋白等致敏大鼠哮喘模型,观察正常组、哮喘组和中药组大鼠支气管肺泡上皮细胞ICAM-1的表达情况及支气管肺泡灌洗液(BALF)中IL-5含量的变化,以及二者之间的关系。结果:与对照组比哮喘组支气管上皮细胞ICAM-1表达显著增强,BALF中IL-5含量显著性增高;哮喘组BALF中细胞计数显著升高,尤其是嗜酸性粒细胞增多更为显著。将哮喘组中ICAM-1的阳性面积、BALF中IL-5的含量进行相关分析,结果三者显著相关;加味玉屏风散可以显著下调ICAM-1的表达,显著降低BALF中IL-5的含量。结论:ICAM-1和IL-5在哮喘大鼠的气道炎症细胞浸润过程中有很重要的作用,二者在嗜酸性粒细胞的活性、趋化作用及其跨内膜转移等方面有很密切的联系;加味玉屏风散可能通过下调ICAM—1和降低IL-5的含量,改善气道炎症状态。更多还原

【Abstract】 Purost : inquire into the effect and mutual relation of ICAM-1 and IL-1 in the course of asthmatic incidence, and the effect to them by the prescrption and medicine of strengthening spleen and benefiting Vital Qi. method: AdoPt the model White rat sensitizing with OA. Observe the expression of ICAM-1 on the broncho-alveolar epithe-lial cell and change of IL-5 in BALF and mutual relation.Result: Compare with the contrast group. The expression of I-CAM-1 on the bronch-epithelial cell increases apparently in the asthma group. The content of IL-5 in BALF increases strik-ingly. For asthma group, the cell count becomes obviously higher especially the increasing of ecsinphilic gametocyte is mochmore evident. We analyze the positive area in ICAM-1 and the content of IL-5 in BALF, the result is closely related. The prescription and medicine of strengthening spleen and benefiting Vital Qi can notably reduce the expression of ICAM-1 and the contents of IL-5 in BALF.Conclu-sion: ICAM-1 and IL-5 play an importan role in the course of the asthmatic white rat’sair way inflammatory cell infiltration. They have close relation in the activity of eosinophilic granulcyte, in the chemo taxis and intimae transfer etc, The prescription and medicine of strengthening spleen and benefiting Vital Qi can reform the airway state by reduce the contents of ICAM-1 and IL-5.更多还原