【作者】 张素军； 瞿伟菁； 李进；

【Author】 Sujun Zhang, WeijingQu, Jin Li (School of Life Science , East China Normal University, Shanghai 200062,China)

【机构】 华东师范大学生命科学学院；

【摘要】 通过PCR产物直接测序,分别获得了硬蒺藜(Tribulus terrestris L．)和软蒺藜(Atriplex centralasiatica Iljin)的核糖体RNA基因—rDNA内转录间隔区(ribosomal DNA internal transcribed spacer,rDNA—ITS)的序列643 bp和607 bp,其碱基总差异率为36．16％,其中,ITS1的碱基差异率为55．81％; 5．8S的碱基差异率为6．59％; ITS2的碱基差异率为56．77％。这种差异,为二者在分子水平上的区分和鉴别提供了DNA指纹谱。更多还原

【Abstract】 To distinguish YINGJILI(Tribulus terrestris L.) from RUANJIL[(Atriplex centralasiatica Iljin) under molecular conditions, which are sometimes used unidentified in traditional Chinese medicine, the nucleotide sequences of ribosomal DNA internal transcribed spacer(rDNA-ITS) of each were achieved using polymerase chain reaction(PCR) followed by an immediate determination of sequences of PCR amplifications. 643 bp nucleotides of ITS of YINGJILI were attained which show a 36.16% diversity compared to 607bp nucleotides of RUANJILI. Meanwhile, the sequences of ITS 1 and ITS2 have the diversities of 55.81% and 56.77%, respectively, while 5.8S showing 6.59%. Therefore, all of the diversities ,as well as the ITS sequence per se, guarantee a DNA fingerprint for identification of YINGJILI and RUANJILI.更多还原