大孔吸附树脂分离羊踯躅根中总黄酮的研究

【作者】 张长弓； 程明； 雷玉霞； 龚雯；

【机构】 华中科技大学同济药学院生药教研室；

【摘要】 目的筛选适合分离纯化羊踯躅根中总黄酮的大孔吸附树脂,确定吸附洗脱参数,选择最佳组合富集羊踯躅根中黄酮的含量。方法以吸附容量、吸附率、解吸附率及吸附动力学特征为考察指标对4种大孔吸附树脂进行综合评定。结果4种大孔吸附树脂对样品溶液中总黄酮的静态吸附容量及吸附率的结果有: SP-825>DM-301>D-101>AB-8;其解吸附率的结果有:D-101>SP-825>AB-8>DM-301。结论在所选树脂中以SP-825吸附与洗脱效果最佳。更多还原

【Abstract】 OBJECTIVE To select the suitable resin to separate and purify the total flavones from the root of Rhododendron and establish the optimum technological parameters of purification. METHODS Four types of macroreticular resin were used to separate and purify total flavones. RESULTS The static adsorption capacity of types of macroreticular resin was SP-825>DM-301>D-101>AB-8; and the adsorption ratio was D-101> SP-825> AB-8> DM-301. CONCLUSION The SP-825 type of macroreticular resin showed the better comprehensive property and could be used to separate and purify the total flavones from Rhododendron .更多还原