【作者】 陈建真； 陈建明； 罗小敏； 叶磊；

【Author】 Chen Jianzhen Chen Jianming Luo Xiaomin Ye Lei (Zhejiang College of TCM, Hangzhou 310053) Zhejiang Province Hospital of TCM ,Hangzhou 310006)

【机构】 浙江中医学院药学系； 浙江省中医院；

【摘要】 目的制定复方黄芩口服液的质量质量标准。方法采用薄层色谱法定性鉴别黄芩、芍药和甘草,用高效液相色谱法测定制剂中黄芩苷的含量。以C18化学键合硅胶为固定相,以甲醇-水-磷酸(50:50:0．3)为流动相,检测波长为280nm。结果定性鉴别色谱特征明显,重现性好。黄芩苷在9．6-57．6ug/mL 范围内呈良好的线性关系,r=0．9998,平均回收率为98．62％,RSD=1．65％。结论上述方法可靠、实用,可有效地控制该制剂的质量。更多还原

【Abstract】 OBJECTIVE To establish the quality standard of Fufang Huangqin Oral liquid. METHOD Radix Scutellariae,Radix Paeoniae Alba and Radix Glycyrrhizae were identified by TLC. The content of baicalin was determined by HPLC,with C18 chemical bonded silica gel as solid phase and methanol-water- phosphoric acid (50: 50: 0.3) as mobile phase.UV detection wavelength was at 280 nm.RESULTS The characteristics for identification by TLC were distinct.The linear range was9.6-57.6ug/mL,r=0.9998.The average recovery of baicalin was 98.62%.RSD was 1.65%.CONCLUSION The methods above-mentioned were reproducible and realizable,and can effectively control the quality of Fufang Huangqin Oral liquid.更多还原