【作者】 李慧； 王弘； 陈世忠；

【Author】 LI Hui, WANG Hong, CHEN Shi-Zhong (School of Pharmacology, Peking University, Beijing 100083, China)

【机构】 北京大学药学院；

【摘要】 提出了一种用反相高效液相色谱直接测定术类药材中苍术酮含量的方法, 一种使苍术酮保持稳定的方法。方法高效液相采用YWG-C18柱,流动相为乙腈．水-磷酸(77:23:0．2,体积比):检测波长为220 nm．;柱温为40．0℃。样品溶液中的苍术酮经充氮气后保持稳定。结果苍术酮标准曲线的线性范围为32．6-293．8 mg·L-1。平均加样回收率为98．4％(n=5)。结论与以往的检测方法相比,该方法稳定,准确,灵敏,简便,适用于白术及其炮制品、茅苍术、北苍术及关苍术的质量控制。更多还原

【Abstract】 A RP -HPLC method for derect determination of the content of Atractylone in Atractylodes and a method to keep Atractylone stable was established . METHOD HPLC was performed on YWG-C18 column with CH3CN - H2O - H3PO4 ( 77:23:0.2 , v/v )as mobile phase. The detection wavelength was 220nm.The temperature of the colume was 40.0μ . The Atractylone in sample solution was kept stable in nitrogen. RESULT The linear range of Atractylone on calibration curve was 32.6-293.8 mg· L- 1 .The average recovery of Atractylone was 98.4% (n=5) . CONCLUSION Compared with the bypast determining method, the method was stable , exact , accurate and simple . It can be used for the quality control of Atractylodes macrocephala and their processed products, A. lancea (Thunb.) DC. , A. chinensis (DC.) Koidz, and the A. japonica koidz.ex Kitam.更多还原