【作者】 陈体强； 吴锦忠； 徐洁；

【Author】 CHEN Ti-qiang, WU Jin-zhcng, XU Jie

【机构】 福建省农业科学院； 福州大学化学与化工学院；

【摘要】 本研究提出的γ-氨基丁酸测定前处理一种改良方法,在内标试验中的回收率高,可达98．48％,比采用常规酸解法(GB／T 18246-2000)提高了12-13．76％, 比乙醇回流-水提法提高7．00-7．39％。在一些植物干鲜品γ-氨基丁酸测定中,采用该方法取得较理想的结果。其中,鲜木瓜果肉中含量高达1．786mg／g,鲜龙眼果肉中含量也较高(1．244-1．325 mg／100g)。干样中,麦芽的γ-氨基丁酸含量较高,达 0．623mg／g,其次为稻芽0．579-0．595mg／g和红(米曲)(0．419-0．489mg／g),蔓荆子、绿豆、莲子等中也含有一定量的γ-氨基丁酸;此外,经炮制后的炒麦芽和炒谷芽, 其γ-氨基丁酸含量降低过半。更多还原

【Abstract】 In this paper, the pretreatment methods of analysis on γ-Amino butyric acid(GABA)were compared, using HITACHI 835-50 type Amino Acid Analyier by standard analysis procedure of 72min ).The result indicated: the adopted improved method was preferable, the recovery is increasing respectively 12-13.76% and 7.00-7.39% then common method: acid hydrolyzation(GB/T 18246-2000) and ethanol circumfluence extraction, and the recovery was validated up to 98.48% by internal standard method. Among some fresh plants products, the GABA contents in papaya(1.786mg/g)was higher while there was 1.244-1.325 mg/100g in longan sarcocarp(arillus longan). And among some dry plants products, the GABA contents in wheat germ (fructus hordei germinatus) was higher upto 0.623mg/g, and the following successively were gemmiparous paddy(fructus oryzae germinatus, 0.572-0.595mg/g) and red kojic rice (0.419-0.489 mg/g). There are also some GABA in fructus viticis(0.316mg/g), mung bean(0.340mg/g) and semein nelumbinis(0.276 mg/g). After parch-processing, the contents in saute wheat germ and saute gemmiparous paddy(0.209 mg/g) were decreased over a half.更多还原