【作者】 张琦； 赵因；

【Author】 Zhang Qi, Zhao Ying (Sichuan Enwei Traditional Chinese Medicine Institute chengdu 610041)

【机构】 四川恩威中医药研究所；

【摘要】 目的:采用高效毛细管电泳法的CZE模式分离测定正在研制的三类新药—清经胶囊（已获临床批文）中小檗碱含量。方法:电泳分离条件:毛细管柱:50cm（有效长度）×75um;运行缓冲液:0.2M乙酸钠;甲醇=8:2;电动方式200mbar·s压力进样;运行电压15kv;柱上检测λ=265nm;毛细管柱温25℃。结果:小檗碱进样浓度在9.95～159.2mg·L^-1范围内线性关系良好（r=0.9999）,平均加样回收率为100.38％（RSD=1.86％）。结论:该法可用于清经胶囊中小檗碱的质量控制。更多还原

【Abstract】 PURPOSE: To establish method for determination of berberine in Qinjin Jiaonang by HPCE. METHODS: capillary zone electrophoresis separation condition: column: fused-silica capillary column(58cm×75um I.D.); running buffer: 0.2M sodium acetate solution : methanol (8: 2) ; injection: 200mbars, detection wave-length : 265nm. column temperature: 25℃. RESULTS: Within the range of 9.95～159.2μg/ml berberine kept a fine line relationship between amount and peak area rate (r=0.9999). The average recovery in sample was 100.38%(n=5, RSD=1.86%). CONCLUTION: This method could be used for quality control of berberine from Qingjing Jiaonang.更多还原