【作者】 胡军祥； 杨琼霞； 姜玉新；

【Author】 Hu Junxiang Yang Qiongxia Jiang Yuxin College of Forestry and Biotechnology, Zhejiang Forestry University, Lin’ an, Zhejiang 311300; Institute of Biotechnology, Zhejiang University. Hangzhou 310029

【机构】 浙江林学院林业与生物技术学院； 浙江大学生物技术研究所；

【摘要】 对ICR小鼠骨髓有核细胞进行玻璃化深低温保存,在冻存1、5、10、20、30d后,骨髓有核细胞样品分别采用常规的38℃水浴复温和微波复温即在300mL 10℃水中,控制微波功率为 800W,频率2450MHz,复温时间80s。检测骨髓有核细胞的功能变化,比较两种复温方法对细胞活性的影响。结果表明:骨髓有核细胞玻璃化冻存1-20d,其存活率、细胞内琥珀酸脱氢酶(SDH)、超氧化物歧化酶(SOD)活性都有下降的趋势,过氧化氢(H2O2)含量显著增加,但冻存20d后这些指标保持基本稳定,与第30d比较无显著差异(P>0．05)。微波复温组的细胞存活率,细胞内 SDH、SOD的活性高于水浴复温组,细胞内H2O2含量明显低于水浴复温组。说明微波复温能够提高复温速率,对骨髓有核细胞的损伤要低于水浴复温。更多还原

【Abstract】 The ICR mice bone marrow nucleated cells(BMNC) were cryopreserved by vitrification.After cryopreservation for 1,5,10,20 and 30 days, the individual samples were separately thawed in 38℃ water bath or by microwave treatment in 300mL 10℃ water under microwave power of 800W, frequency of 2450MHz and thawing duration of 80s, following which the viability of BMNC was assayed.The experimental results showed that during cryopreservation of 1-20days, the survival rate of the cells,and the activity of succinate dehydrogenase (SDH) and superoxide dismutase (SOD) of BMNC were significantly depressed, whereas the hydrogen peroxide (H2O2) content of BMNC was markedly increased.After cryopreservation for 20 days these indexes remained relatively stable so that no significant difference (P>0.05) was found between in these indexes before and after being preserved for 30 days.The cell survival rate, the activity of SDH and SOD of BMNC subjected to microwave thawing were higher than those of BMNC subjected to water bath thawing, whereas the H2O2 content of BMNC of the former was lower than that of the latter.It was indicated that damage to the microwave-thawed BMNC was lighter than damage to water bath-thawed BMNC.更多还原