【作者】 孟凡会； 孙青刚； 刘竹珍； 潭金山； 刘金兰；

【Author】 Meng Fanhui, Sun Qinggang, Wang tao, et al. Medical School Hospital of Qingdao University, 266003

【机构】 青岛大学医学院附属医院；

【摘要】 目的观察体积分数0．02二甲亚砜(DMSO)联合第二信使效应剂(ThromboSol)对深低温保存浓缩血小板超微结构的影响。方法分别应用3种不同的方法(体积分数0．02DMSO、体积分数0．06DMSO、体积分数0．02DMSO+ThromboSol)在-196℃冷冻保存浓缩血小板,并在冻存后1、6个月时分别取出复温,采用扫描和透射电镜观察血小板超微结构变化。结果体积分数 0．02DMSO+ThromboSol组血小板能保持完整结构,部分血小板出现激活迹象,冷冻保存1个月和 6个月的血小板超微结构无明显差异。结论体积分数0．02DMSO+ThromboSol能够有效地保护血小板超微结构,其6个月内的保存效果稳定。更多还原

【Abstract】 Objective To observe the effect on platelet ultrastructure after cryopreservation using 2% dimethyl sulfoxide(DMSO) plus ThromboSol, a mixture of second - messenger effectors.Methods Platelet concentrates were treated with 2% DMSO,0.06%DMSO and 2% DMSO plus ThromboSol,then placed in liquid nitrogen.After frozen for 1 month and 6 months, samples were thawed and their ultrastructure was observed by SEM and TEM..Results The results of SEM and TEM showed that the platelets cryopreserved by 2% DMSO plus ThromboSol could retain good ultrastructure relatively and that a part of platelets was in active statue.There was no significantly differences between 1 month group and 6 month group.Conclusion 2%DMSO plus ThromboSol might protect effectively the platelet ultrastructure and the long-term cryopreservative effect was stable.更多还原