【作者】 李世刚； 张永琦； 赵健雄； 王学习； 刘忠；

【Author】 Li Shigang Zhang Yongqi Zhao Jianxiong (Institute of Integrated Traditional Chinese Medicine and Western Medicine,College of Basic Medicine,Lanzhou University,Lanzhou 730000)

【机构】 兰州大学基础医学院中西医结合研究所；

【摘要】 目的：研究红芪多糖均一组分 HPS-3体外对人胃腺癌 MGC-803细胞增殖及凋亡的作用。方法：应用 MTT 法、显微荧光术和流式细胞术测定 HPS-3对 MGC-803细胞的增殖、细胞周期和诱导凋亡的影响,应用流式细胞术测定对 Bcl-2和 Bax 蛋白表达的影响。结果：HPS-3对 MGC-803细胞具有抑制增殖、G2/M 期阻滞和诱导凋亡作用；HPS-3降低 bcl-2蛋白表达,对 Bax 蛋白表达无明显作用。结论：HPS-3可通过抑制人胃腺癌 MGC-803细胞的生长增殖、G2/M 期阻滞、诱导细胞凋亡,降低 bcl-2蛋白等作用机制发挥肿瘤作用。更多还原

【Abstract】 Object:To study the effect of Hedysari polysaccharide HPS-3 on the proliferation and apoptosis of human gastric cancer cell line MGC -803 in vitro.Methods:MTT assay,flow cytometry and fluorescence microscopy were used to examine the effects of HPS-3 in anti-pro- liferation,cell cycle arresting and apoptosis induction.Flow cytometry were used to detect the effects on protein expression of Bcl-2 and Bax.Results:HPS-3 could cause apparent inhibition on cell growth,induce G2/M phase arrest and apoptosis on MGC-803 cells.HPS- 3 could down-regulate bcl-2 protein expression,but Bax protein expression showed unchanged.Conclusion:HPS-3 shows its anti- tumor effects by way of inhibiting the cell growth,arresting the G2/M phase,inducing apoptosis,down-regulating bcl-2 protein expres- sion.更多还原