【作者】 胡成穆； 姜辉； 刘洪峰； 李荣； 李俊；

【Author】 Hu Chengmu Jiang Hui Liu Hongfeng Li Rong Li Jun (School of Pharmacy,Anhui Medical University,Hefei 230032)

【机构】 安徽医科大学药学院；

【摘要】 目的：观察金银花总黄酮(Lonicera gaponica total flavone,LJTF)对卡介苗(BCG)联合脂多糖(LPS)所致小鼠免疫性肝损伤的保护作用。方法：采用 BCG 2．5mg 静脉注射致敏小鼠,d10给于 LPS 7．5μg 静脉攻击造成小鼠免疫性肝损伤模型,ig 给于 LJTF(100、200、400mg/kg)连续10天。分光光度法测定血清 ALT、AST 浓度,肝匀浆中 MDA、SOD 的含量；免疫组化研究肝脏肿瘤坏死因子-α(TNF-α)和核因子(NF)-κB p65的表达。结果：免疫性肝损伤小鼠血清 ALT、AST 较正常组明显升高,肝脏中 MDA 明显升高而 SOD 较正常组明显降低。LJTF ig 能降低免疫性肝损伤小鼠血清中升高的 ALT、AST 含量,降低肝匀浆中 MDA 水平,同时升高其低下的 SOD 水平,LJTF 也能降低免疫性肝损伤小鼠 TNF-α和 NF-κB p65在肝脏中的强烈表达。结论：LJTF 对免疫性肝损伤小鼠有保护作用,机制与减少自由基的产生、抑制细胞膜脂质过氧化、减少炎症介质的释放有关。更多还原

【Abstract】 Objective:To study the protective effect of LJTF on immunological liver injury in mice.Methods:The model of mouse immunological liver injury was induced by injection of Bacillus Calmette Guerin(BCG)and lipopolysaccharide(LPS)from tail vein.The alanine amin- otransferase(ALT)、aspartate aminotransferase(AST)level in serum and alondiadehyde(MDA)content,superoxide dismutase(SOD)ac- tivities in liver were assayed by spectrophotometry.TNF-α and NF-κB p65 in hepatic tissues was also determined by the method of immu- nohistochemistry to evaluate the protective effect of LJTF.Results:Immunological liver injury was successfully induced by BCG+LPS.LJTF (100,200,400 mg/kg) ig×10d significantly decreased ALT、AST activity in serum,reduced liver MDA content in immunological liver injury mice.LJTF also improved the reduced SOD level in liver homogenate.Further more,LJTF could inhibit the express of TNF-α and NF-κB p65 in liver tissue.Conclusion:LJTF showed significantly protective action on immunological liver injury in mice.Its mechanism possibly due to deplete ORF,suppressor cell lipid peruxidation and diminish mediators of inflammation.更多还原