【作者】 熊杰； 徐平湘； 孙丽娜； 成亮； 冯亦璞； 王晓良；

【Author】 Xiong Jie~1 Xu Pingxiang~1 Sun lina~1 Cheng Liang~1 Feng Yipu~2 Wang Xiaoliang~2 (~1 Department of Pharmacology,School of Chemical Biology and Pharmacal Science,Capital Medical University,Beijing 100069;~2 Institute of Materia Medica,Chinese Acedimy of Medical Sciences·Peking Union Medical Colloge,Beijing 100050)

【机构】 首都医科大学化学生物学与药学院药理系； 中国医学科学院·中国协和医科大学药物研究所；

【摘要】 探讨丁基苯酞（butylphthalide,NBP）对原代培养神经元在低糖低氧损伤下的保护作用及线粒体作用机制。方法：采用 Hoechst 33342和 PI 共染的方法,观察 NBP 对低糖低氧损伤造成神经细胞坏死和凋亡的影响,并用荧光探针标记以及分光光度法检测 NBP 对神经细胞线粒体膜电位、线粒体膜流动性及线粒体呼吸链复合酶 IV 活性的影响。结果：NBP(10^-6～10^-4 mol/L)能显著减少低糖低氧引起的神经细胞坏死和凋亡；机制研究表明它能明显改善由损伤引起的神经细胞线粒体膜电位、线粒体膜流动性及线粒体呼吸链复合酶 IV 活性的降低。结论：NBP 对低糖低氧损伤导致的神经细胞坏死和凋亡具有良好的保护作用, 而线粒体保护可能是其作用机制之一。更多还原

【Abstract】 Objective:To investigate the protective effects of butylphthalide (NBP) on neuronal necrosis and apoptosis caused by hypoxia/hypogly- caemia,and its mitochondrial mechanisms.Methods:Dual stain with Hoechest 33342 and Propidium Iodide (PI) was used to detect the necrosis and apoptosis.Rhodamine-123 (rhod-123) and DPH were used as fluorescent indicators to measure the mitochondria membrane potential (MMP) and the mitochondria membrane fluidity (MFu).The activity of complex Ⅳ of respiratory chain was detected by spectro- photometry.Results:NBP could remarkably reduce the neuronal necrosis and apoptosis caused by hypoxia/hypoglycemia.It could increase the MMP,MFu and the activity of complex Ⅳ,which were dropped during the hypoxia/hypoglycemia injury.Conclusions:NBP could pro- tect cultured neurons against hypoxia/hypoglycemia injury,and the mitochondria protection is involved in its mechanism.更多还原