【作者】 武继彪； 隋在云； 管华诗；

【Author】 Wu Jibiao~1 Sui Zaiyun~2 Guan Huashi~1 (~1 School of Medicine,China Ocean University,Qingdao 266003;~2 Shandong Academy of TCM,Jinan 250014)

【机构】 中国海洋大学； 山东省中医药研究院；

【摘要】 目的：研究丹皮酚对大鼠海马神经元缺糖缺氧的保护作用。方法：以原代培养的新生大鼠海马神经元作为研究对象,建立缺糖缺氧损伤模型,倒置相差显微镜下进行一般形态学的动态观察,用 MTT 法测定神经元存活率。通过放射配基结合实验测定海马 NMDA 受体结合力变化。结果：丹皮酚可显著降低神经元缺糖缺氧时细胞死亡率,减弱 NMDA 受体结合力。结论：丹皮酚对离体培养的大鼠海马神经元缺糖缺氧具有保护作用,其作用机制与减弱 NMDA 受体结合力有关。更多还原

【Abstract】 Objective:To determine if peaonol can protect cultured rat hippocampal neurons from anoxia-induced injury.Methods:We cultivate the rat hippocampal neurons as the object of study and then build the model of oxygen-glucose deprivation.They were randomly divided into 3 groups.Each group was observed by inverted phase contrast microscope;bonding force of NMDA receptors was evaluated by liquid scintilla- tion counting.Results:Compared with OGD group at each designated period,peaonol treatment after OGD obviously increased cell survival rate and reduced the bonding force of NMDA receptors.Conclusion:Peaonol can protect rat neurons from ischemia-reperfusion injury by al- leviating morphological damage and increasing neuron viability.更多还原